Caspase-6 Inhibitor Screening Kit (Fluorometric)

• Cat.No.: Kit-2330
• Product Overview: Caspase-6 Inhibitor Screening Assay Kit provides a robust and easy-to-use platform for identifying novel inhibitors of human caspase-6, a cysteine protease implicated in multiple neurodegenerative disorders. The assay uses a caspase-6-specific fluorogenic substrate, Z-VEID-AFC. Caspase-6 cleaves this substrate generating free AFC, which can be easily quantified using a fluorescence plate reader at excitation and emission wavelengths of 400 and 505 nm, respectively. The potent and reversible caspase-6 inhibitor Ac-VEID-CHO is included as a positive control and inhibits caspase-6 with an IC₅₀ value of 12 nM.

Specification

• Size: 384 wells
• Storage: Kit will arrive packaged as a -80°C kit. After opening kit, store individual components as stated below.
• Shipping: Dry ice
• Kit Components: Caspase Assay Buffer (5X): 1 vial/5 ml, -20°C. DTT (1 M) Assay Reagent: 1 vial/1 ml, -20°C. Caspase-6 Substrate (Z-VEID-AFC): 1 vial/50 μl, -20°C. Caspase-6 Enzyme (human, recombinant): 1 vial/15 μl, -80°C. Caspase-6 Inhibitor (Ac-VEID-CHO): 1 vial/25 μl, -20°C. 384-Well Solid Plate (low volume; black): 1 plate, RT. Foil Plate Cover: 1 ea, RT.
• Materials Required but Not Supplied: 1. A plate reader with the ability to measure fluorescence with excitation and emission wavelengths of 400 and 505 nm, respectively 2. Adjustable pipettes and a multichannel pipette 3. A source of pure water; glass-distilled water or deionized water is acceptable