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Cathepsin S Inhibitor Screening Kit (Fluorometric)

Cat.No.: Kit-2353
Product Overview: Cathepsin S (CTSS, EC 3.4.22.27) is a lysosomal cysteine proteinase that is suggested to participate in the degradation of antigenic proteins to peptides for presentation on MHC class II molecules. Cathepsin S Inhibitor Screening Kit utilizes the ability of Cathepsin S to cleave the synthetic AFC based peptide substrate to release AFC, which can be easily quantified using a fluorometer or fluorescence microplate reader. In the presence of a Cathepsin S inhibitor, the cleavage of the substrate is reduced/abolished resulting in decrease or total loss of the AFC fluorescence. This high-throughput adaptable assay kit is simple, sensitive, and rapid tool to screen the potential inhibitors of Cathepsin S.
Applications: Screen/study/characterize potential inhibitors of Cathepsin S.
Storage: -20°C
Shipping: Gel Pack
Size: 100 assays
Kit Components: CTSS Reaction Buffer; Cathepsin S (human); CTSS Substrate, Z-VVR-AFC (10 mM); CTSS Inhibitor (Z-FF-FMK, 1 mM)
Detection method: Fluorescence (Ex/Em = 400/505 nm)
Features & Benefits: Simple & convenient;
High-throughput adaptable assay to screen/study/characterize the potential inhibitors of Cathepsin S;
Cathepsin S inhibitor, Z-FF-FMK is included in the kit

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