"Cdc42" Related Products


Cdc42 G-LISA Activation Assay Kit (Colorimetric)

Cat.No.: Kit-0190
Product Overview: The G-LISA series of Small G-Protein Activation Assays are ELISA based assays with which you can measure the GTP form of small G-proteins from lysates of cells or tissues and all in less than 3 h. The Cdc42 G-LISA Activation Assay measures the entire level of GTP-loaded Cdc42 protein in cell lysates. The level of activation is measured by reading at OD490nm. For a more detailed introduction on G-LISA assays and a listing of other available G-LISA kits, see our main G-LISA page. For a kit to measure RhoA activation please check webpage BK124, for Rac1 go to BK128 for Rac1,2,3 go to BK125, for RalA go to BK129.The Cdc42 G-LISA Activation Assay is very sensitive and has excellent accuracy for duplicate samples.
Size: 24 assays
Applications: 1. Cdc42 signaling pathway studies.2. Cdc42 activation assays with primary cells.3. Studies of Cdc42 activators and inactivators.4. Cdc42 activation assays with limited material.5. High throughput screens for Cdc42 activation.
Kit Components: Reagent-Quantity-StorageCdc42-GTP binding strips: 3 strips of 8 wells, Desiccated 4°C; Anti-Cdc42 antibody (mouse monoclonal): 1 tube, lyophilized, Desiccated 4°C; Secondary antibody-horseradish peroxidase conjugate (HRP): 1 tube, lyophilized, Desiccated 4°C; Cdc42 control protein (constitutively active Cdc42): 1 tube, lyophilized, Desiccated 4°C; CDCA Resuspension Buffer: 1 tube, liquid, 4°C; Cell Lysis Buffer: 1 bottle, lyophilized, Desiccated 4°C; Wash Buffer Part: 1 bottle, lyophilized, Desiccated 4°C; Antigen Presenting Buffer: 1 bottle, 10 ml, Room temperature; Antibody Dilution Buffer 1 bottle, lyophilized, Desiccated 4°C; HRP Detection Reagent A: 1 tablet, silver pack, Desiccated 4°C; HRP Detection Reagent B: 1 tablet, gold pack, Desiccated 4°C; HRP Reagent Stop Solution: 1 bottle, 8 ml 4°C; Precision Red Advanced Protein Assay Reagent: (available as 500 ml size Cat. ADV02), 1 bottle, 30 ml Room temperature; Protease Inhibitor Cocktail: 1 tube, lyophilized, Desiccated 4°C

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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