Recombinant Human Argininosuccinate Synthase 1, His-tagged

• Cat.No.: ASS1-777H
• Product Overview: Recombinant human ASS1 protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques. MW =48.6 kDa (432aa) confirmed by MALDI-TOF.

Specification

• Cat. No.: ASS1-777H
• Description: The ASS1 is an enzyme called argininosuccinate synthase 1. This enzyme participates in the urea cycle, which is a sequence of chemical reactions that takes place in liver cells. The urea cycle processes excess nitrogen that is generated as the body uses proteins. The excess nitrogen is used to make a compound called urea, which is excreted from the body in urine.
• Source: E.coli.
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MSSKGSVVLA YSGGLDTSCI LVWLKEQGYD VIAYLANIGQ KEDFEEARKK ALKLGAKKVF IEDVSREFVEEFIWPAIQSS ALYEDRYLLG TSLARPCIAR KQVEIAQREG AKYVSHGATG KGNDQVRFEL SCYSLAPQIK VIAPWRMPEF YNRFKGRNDL MEYAKQHGIP IPVTPKNPWS MDENLMHISY EAGILENPKN QAPPGLYTKT QDPAKAPNTP DILEIEFKKG VPVKVTNVKD GTTHQTSLELFMYLNEVAGK HGVGRIDIVE NRFIGMKSRG IYETPAGTIL YHAHLDIEAF TMDREVRKIK QGLGLKFAEL VYTGFWHSPE CEFVRHCIAK SQERVEGKVQ VSVLKGQVYI LGRESPLSLY NEELVSMNVQ GDYEPTDATG FININSLRLK EYHRLQSKVT AK
• Purity: > 90% by SDS – PAGE.
• Form: Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 20% glycerol 0.1M NaCl,1mM DTT.
• Concentration: 0.5 mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: ASS1 argininosuccinate synthase 1 [ Homo sapiens ]
• Synonyms: ASS1; argininosuccinate synthase 1; ASS; CTLN1; argininosuccinate synthase; OTTHUMP00000022363; OTTHUMP00000022364; citrulline-aspartate ligase; citrulline--aspartate ligase; argininosuccinate synthetase 1; EC 6.3.4.5; argininosuccinate synthetase
• Gene ID: 445
• mRNA Refseq: NM_000050
• Protein Refseq: NP_000041
• MIM: 603470
• UniProt ID: P00966
• Chromosome Location: 9q34.1
• Pathway: Alanine, aspartate and glutamate metabolism; Arginine and proline metabolism; Metabolic pathways
• Function: ATP binding; argininosuccinate synthase activity; ligase activity; nucleotide binding; protein binding

Reviews

09/04/2020

Critical expertise in protein engineering, a trusted research resource.

10/01/2019

Dependable peptide synthesis, accelerates our peptide-based projects effectively.

08/10/2018

Efficient protein purification, cost-effective and dependable.

Q&As

What are the mechanisms by which ASS1 modulates cell growth and proliferation?

ASS1 regulates cell growth by controlling the availability of arginine, a key amino acid for cell proliferation.

How does ASS1 deficiency contribute to the development of urea cycle disorders and hyperammonemia?

ASS1 deficiency leads to urea cycle disorders, causing hyperammonemia and metabolic imbalances.

What is the impact of ASS1 on amino acid metabolism and overall cellular function?

ASS1 affects amino acid metabolism, playing a critical role in cellular health and function.

How does ASS1 (Argininosuccinate Synthase 1) function in the urea cycle and nitrogen metabolism?

ASS1 is essential in the urea cycle, converting nitrogen waste into urea for excretion and maintaining nitrogen balance.

How do alterations in ASS1 expression or activity affect cardiovascular health and disease?

Variations in ASS1 activity can impact cardiovascular health, influencing nitric oxide production and vascular function.

What role does ASS1 play in the synthesis of arginine and its impact on nitric oxide production?

ASS1 is involved in arginine synthesis, impacting nitric oxide levels and vascular function.

How does ASS1 influence the response to high-protein diets or dietary restrictions in metabolic health?

ASS1 influences how the body metabolizes high-protein diets, affecting metabolic responses and health.