Recombinant Human Argininosuccinate Synthase 1, His-tagged ASS1-777H

Recombinant Human Argininosuccinate Synthase 1, His-tagged

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Recombinant Human Argininosuccinate Synthase 1, His-tagged

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Cat. No. : ASS1-777H
Product Overview : Recombinant human ASS1 protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques. MW =48.6 kDa (432aa) confirmed by MALDI-TOF.
Description : The ASS1 is an enzyme called argininosuccinate synthase 1. This enzyme participates in the urea cycle, which is a sequence of chemical reactions that takes place in liver cells. The urea cycle processes excess nitrogen that is generated as the body uses proteins. The excess nitrogen is used to make a compound called urea, which is excreted from the body in urine.
Source : E.coli.
Sequences Of Amino Acids : MGSSHHHHHH SSGLVPRGSH MSSKGSVVLA YSGGLDTSCI LVWLKEQGYD VIAYLANIGQ KEDFEEARKK ALKLGAKKVF IEDVSREFVEEFIWPAIQSS ALYEDRYLLG TSLARPCIAR KQVEIAQREG AKYVSHGATG KGNDQVRFEL SCYSLAPQIK VIAPWRMPEF YNRFKGRNDL MEYAKQHGIP IPVTPKNPWS MDENLMHISY EAGILENPKN QAPPGLYTKT QDPAKAPNTP DILEIEFKKG VPVKVTNVKD GTTHQTSLELFMYLNEVAGK HGVGRIDIVE NRFIGMKSRG IYETPAGTIL YHAHLDIEAF TMDREVRKIK QGLGLKFAEL VYTGFWHSPE CEFVRHCIAK SQERVEGKVQ VSVLKGQVYI LGRESPLSLY NEELVSMNVQ GDYEPTDATG FININSLRLK EYHRLQSKVT AK
Purity : > 90% by SDS – PAGE.
Form : Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 20% glycerol 0.1M NaCl,1mM DTT.
Concentration : 0.5 mg/ml (determined by Bradford assay).
Storage : Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
PDB rendering based on 2nz2. :

Gene Name : ASS1 argininosuccinate synthase 1 [ Homo sapiens ]
Synonyms : ASS1; argininosuccinate synthase 1; ASS; CTLN1; argininosuccinate synthase; OTTHUMP00000022363; OTTHUMP00000022364; citrulline-aspartate ligase; citrulline--aspartate ligase; argininosuccinate synthetase 1; EC 6.3.4.5; argininosuccinate synthetase
Gene ID : 445
mRNA Refseq : NM_000050
Protein Refseq : NP_000041
MIM : 603470
UniProt ID : P00966
Chromosome Location : 9q34.1
Pathway : Alanine, aspartate and glutamate metabolism; Arginine and proline metabolism; Metabolic pathways
Function : ATP binding; argininosuccinate synthase activity; ligase activity; nucleotide binding; protein binding

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