||Recombinant human MAP1LC3A protein, fused to His-tag at C-terminus, was expressed inE.coliand purified by using conventional chromatography techniques. MW =15.1 kDa (128aa) confirmed by MALDI-TOF.
||Microtubule-associated proteins 1A/1B light chain 3A isoform a, also known as MAP1LC3A, is one of the light chain subunits and can associate with either MAP1A or MAP1B. It plays critical roles in neuronal development and in maintaining the balance between neuronal plasticity and rigidity. MAP1LC3A is expressed as two alternatively spliced isoforms that are expressed in testis, brain, heart, liver and skeletal muscle, but are absent in thymus and peripheral blood leukocytes.
|Sequences Of Amino Acids:
||MPSDRPFKQR RSFADRCKEV QQIRDQHPSK IPVIIERYKG EKQLPVLDKT KFLVPDHVNM SELVKIIRRR LQLNPTQAFF LLVNQHSMVS VSTPIADIYE QEKDEDGFLY MVYASQETFG LEHHHHHH
||> 95% by SDS – PAGE.
||Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 0.1M NaCl.
||1 mg/ml (determined by Bradford assay).
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.