Recombinant Human APEX1, T7-tagged

• Cat.No.: APEX1-955H
• Product Overview: Recombinant human APEX1 protein, fused to T7-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Cat. No.: APEX1-955H
• Description: APEX1, also known as REF1 or APE1 (Apurinic / apyrimidinic endonuclease), is responsible for the incision of DNA basic sites during base excision repair. It has also been shown to stimulate the DNA binding activity of numerous transcription factors that are involved in cancer promotion and progression. APEX1 is part of the cellular response to oxidative stress and protects cells from the genotoxic and cytotoxic effect of oxidizing agents.
• Source: E.coli.
• Form: Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.2M NaCl, 2mM DTT, 10% glycerol.
• Molecular Weight: 36.9 kDa (332aa) confirmed by MALDI-TOF.
• Purity: > 90% by SDS – PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Sequences Of Amino Acids: MASMTGGQQM GRGSMPKRGK KGAVAEDGDE LRTEPEAKKS KTAAKKNDKE AA GEGPALYE DPPDQKTSPS GKPATLKICS WNVDGLRAWI KKKGLDWVKE EAP DILCLQE TKCSENKLPA ELQELPGLSH QYWSAPSDKE GYSGVGLLSR QCP LKV SYGI GEEEHDQEGR VIVAEFDSFV LVTAYVPNAG RGLVRLEYRQ RWDEAFRKFL KGL ASRKPLV LCGDLNVAHE EIDLRNPKGN KKNAGFTPQE RQGFGELLQA VPL ADSFRHL YPNTPYAYTF WTYMMNARSK NVGWRLDYFL LSHSLLPALC DSK IRSK ALG SDHCPITLYL AL.
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Pathways: Base excision repair; DNA Repair

Gene Information

• Gene Name: APEX1 APEX nuclease (multifunctional DNA repair enzyme) 1 [ Homo sapiens ]
• Synonyms: APEX nuclease (multifunctional DNA repair enzyme) 1; APE; APX; APE1; APEN; APEX; HAP1; REF1; APEX1; DNA-(apurinic or apyrimidinic site) lyase; AP lyase; protein REF-1; redox factor 1; AP endonuclease 1; AP endonuclease class I; apurinic/apyrimidinic exonuclease; multifunctional DNA repair enzyme; apurinic-apyrimidinic endonuclease 1; apurinic/apyrimidinic (abasic) endonuclease; deoxyribonuclease (apurinic or apyrimidinic); EC 4.2.99.18; APEX nuclease (multifunctional DNA repair enzyme);APE-1; REF-1; APEX nuclease 1; APEX nuclease
• Gene ID: 328
• mRNA Refseq: NM_001641
• Protein Refseq: NP_001632
• MIM: 107748
• UniProt ID: P27695
• Chromosome Location: 14q11.2-q12
• Function: 3"-5" exonuclease activity; DNA binding; DNA-(apurinic or apyrimidinic site) lyase activity; endodeoxyribonuclease activity; endonuclease activity; lyase activity; metal ion binding; oxidoreductase activity; phosphodiesterase I activity; phosphoric diester hydrolase activity; protein binding; ribonuclease H activity; transcription coactivator activity; transcription corepressor activity; uracil DNA N-glycosylase activity