||Recombinant human A2LD1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
||A2LD1, also known as gamma-glutamylaminecyclotransferase, is an enzyme that converts gamma-glutamylamines to free amines and 5-oxoproline. It shows high activity toward gamma-glutamyl-epsilon-lysine, derived from the breakdown of fibrin and other proteins cross-linked by transglutaminases. This protein adopts the newly identified cyclotransferase fold, observed in gamma-glutamylcyclotransferase, an enzyme with activity toward gamma-glutamyl-alpha-amino acids.
||Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 0.1M NaCl.
||19.4 kDa (173aa) confirmed by MALDI-TOF.
||> 95% by SDS - PAGE.
||1.0 mg/ml (determined by Bradford assay).
|Sequences Of Amino Acids:
||MGSSHHHHHH SSGLVPRGSH MALVFVYGTL KRGQPNHRVL RDGAHGSAAF RAR GRTLEPY PLVIAGEHNI PWLLHLPGSG RLVEGEVYAVDERMLRFLDD FESC PALYQR TVLRVQLLED RAPGAEEPPA PTAVQCFVYS RATFPPEWAQ LPH HD SYDSE GPHGLRYNPR ENR.
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.