||Recombinant human MESDC2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
||MESDC2, also known as Mesoderm development candidate 2, is required for formation of the primitive streak and mesoderm during embryogenesis. This protein binds to the Wnt co-receptors LRP5 and LRP6 in the endoplasmic reticulum and is required for their proper folding and cell surface expression.
||Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol 0.1M NaCl, 1mM DTT.
||24.9 kDa (222aa) confirmed by MALDI-TOF.
||> 90% by SDS - PAGE.
||1.0 mg/ml (determined by BCA assay).
|Sequences Of Amino Acids:
||MGSSHHHHHH SSGLVPRGSH MAEGSPGTPD ESTPPPRKKK KDIRDYNDAD MA R LLEQWEK DDDIEEGDLP EHKRPSAPVD FSKIDPSKPESILKMTKKGK TLM MF VTVSG SPTEKETEEI TSLWQGSLFN ANYDVQRFIV GSDRAIFMLR DGSYAWEIKD FLVGQDRCAD VTLEGQVYPG KGGGSKEKNK TKQDKGKKKK EGDLKSRSSK EENRAGNKRE DL.
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.