||Recombinant human LCN2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
||Neutrophil gelatinase-associated lipocalin (NGAL), also known as LCN2, belongs to the calycin superfamily and Lipocalin family. LCN2 is an iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. The binding of LCN2 to bacterial siderophores is important in the innate immune response to bacterial infection. Also LCN2 functions as a growth factor. LCN2 is strongly upregulated during inflammation and is upregulated by interleukin 1 (but not TNF alpha) in humans.
||Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 1mM DTT, 50mM NaCl.
||22.8kDa (199aa) confirmed by MALDI-TOF.
||> 90% by SDS – PAGE.
||1mg/ml (determined by Bradford assay).
|Sequences Of Amino Acids:
||MGSSHHHHHH SSGLVPRGSH MQDSTSDLIP APPLSKVPLQ QNFQDNQFQG KW YVVGLAGN AILREDKDPQ KMYATIYELK EDKSYNVTSV LFRKKKCDYW IRTFV PGCQP GEFTLGNIKS YPGLTSYLVR VVSTNYNQHA MVFFKKVSQN REYFKITLYG RTKELTSELK ENFIRFSKSL GLPENHIVFP VPIDQCIDG.
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.