Recombinant Human TFAM, His-tagged

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Recombinant Human TFAM, His-tagged

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Cat. No. : TFAM-3416H
Product Overview : Recombinant human TFAM protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Description : TFAM, also known as MtTF1 or mtTFA, is a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. TFAM is located primarily in the nuclei of elongated spermatids and may be involved in the regulation of gene expression of the haploid male genome. TFAM has been associated with mitochondrial disorder in humans characterized by ocular myopathy, exercise intolerance and muscle wasting.
Source : E.coli.
Form : Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.2M NaCl, 5mM DTT, 20% glycerol.
Molecular Weight : 26.6 kDa (225aa) confirmed by MALDI-TOF.
Purity : > 85% by SDS - PAGE.
Concentration : 0.5 mg/ml (determined by Bradford assay).
Sequences Of Amino Acids : MGSSHHHHHH SSGLVPRGSH MSSVLASCPK KPVSSYLRFS KEQLPIFKAQ NP DA KTTELI RRIAQRWREL PDSKKKIYQD AYRAEWQVYK EEISRFKEQL TPS QI MS LEK EIMDKHLKRK AMTKKKELTL LGKPKRPRSA YNVYVAERFQ EAK GD SP QEK LK TVKENWKN LSDSEKELYI QHAKEDETRY HNEMKSWEEQ MIEVGRKDLL RRT IKKQRKY GAEEC.
Storage : Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Pathways : Huntington"s disease; Transcription
Gene Name : TFAM transcription factor A, mitochondrial [ Homo sapiens ]
Synonyms : transcription factor A, mitochondrial; TCF6; MtTF1; mtTFA; TCF6L1; TCF6- L2; TCF6L3; TFAM; transcription factor A, mitochondrial; TCF-6; OTTHUMP00- 000019632; transcription factor 6; mitochondrial transcription factor 1; mitochondrial transcription factor A; Transcription factor 6-like 2 (mitocho- ndrial transcription factor); Mitochond- rial transcription factor 1; Transcription factor 6; Transcription factor 6-like 2; MTTF1
Gene ID : 7019
mRNA Refseq : NM_003201
Protein Refseq : NP_003192
MIM : 600438
UniProt ID : Q00059
Chromosome Location : 10q21
Function : DNA binding; protein binding; sequence-specific DNA binding transcription factor activity

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