Recombinant Human AK4, His-tagged AK4-3448H

Recombinant Human AK4, His-tagged

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Recombinant Human AK4, His-tagged

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Cat. No. : AK4-3448H
Product Overview : Recombinant human AK4 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Description : AK4(adenylate kinase 4) is a member of the adenylate kinase family of enzymes. This protein is localized to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Inherited mutations leading to AK4 deficiencies in erythrocytes have been implicated in hemolytic anemia.
Form : Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl.
Molecular Weight : 27.4 kDa (243aa), confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appear higher)
Purity : > 90 % by SDS - PAGE
Concentration : 1 mg/ml (determined by Bradford assay)
Sequences of amino acids : MGSSHHHHHH SSGLVPRGSH MASKLLRAVI LGPPGSGKGT VCQRIAQNFG LQHLSSGHFL RENIKASTEV GEMAKQYIEK SLLVPDHVIT RLMMSELENR RGQHWLLDGF PRTLGQAEAL DKICEVDLVI SLNIPFETLK DRLSRRWIHP PSGRVYNLDF NPPHVHGIDD VTGEPLVQQE DDKPEAVAAR LRQYKDVAKP VIELYKSRGV LHQFSGTETN KIWPYVYTLF SNKITPIQSK EAY
Storage : Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
PDBrendering based on 2ar7 :

Gene Name : AK4 adenylate kinase 4 [ Homo sapiens ]
Synonyms : AK3L1; adenylate kinase 3-like 1; AK3L1 adenylate kinase 3-like 1; AK3; AK4; MGC166959; EC 2.7.4.3; OTTHUMP00000010594; ATP-AMP transphosphorylase; GTP:AMP phosphotransferase; Adenylate kinase 3-like; adenylate kinase 3; mitochondrial adenylate kinase-3; nucleoside-triphosphate-adenylate kinase
Gene ID : 205
mRNA Refseq : NM_001005353
Protein Refseq : NP_001005353
MIM : 103030
UniProt ID : P27144
Chromosome Location : 1p31.3
Pathway : Purine metabolism
Function : ATP binding; GTP binding; adenylate kinase activity; nucleotide binding; transferase activity

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