||Recombinant human PEF1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
||Penta-EF-hand domain containing 1, also known as PEF1, belongs to the penta-EF-hand protein family, which shares similarities to the apoptosis linked gene ALG-2. PEF1 heterodimerizes with PDCD6/ALG2 and dissociates from PDCD6/ALG2 in the presence of Ca (2+). Also, PEF1 proteins bind calcium and participate in a variety of calcium-dependent processes in vertebrates.
||Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 50% glycerol, 0.2M NaCl
||34.5 kDa (320aa), confirmed by MALDI-TOF
||> 85% by SDS - PAGE
||0.25 mg/ml (determined by Bradford assay)
|Sequences of amino acids:
||MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMASY PYRQGCPGAA GQAPGAPPGS YYPGPPNSGG QYGSGLPPGG GYGGPAPGGP YGPPAGGGPY GHPNPGMFPS GTPGGPYGGA APGGPYGQPP PSSYGAQQPG LYGQGGAPPN VDPEAYSWFQ SVDSDHSGYI SMKELKQALV NCNWSSFNDE TCLMMINMFD KTKSGRIDVY GFSALWKFIQ QWKNLFQQYD RDRSGSISYT ELQQALSQMG YNLSPQFTQL LVSRYCPRSA NPAMQLDRFI QVCTQLQVLT EAFREKDTAV QGNIRLSFED FVTMTASRML
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.