Recombinant Human PPARG, 209-477 aa, His-tagged

• Cat.No.: PPARG-2772H
• Product Overview: Recombinant human PPARG protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques. MW = 33.0kDa (290aa).

Specification

• Cat. No.: PPARG-2772H
• Description: PPARG, also known as Peroxisome proliferator-activated receptor gamma, is member of the nuclear hormone receptor subfamily of transcription factors. This protein regulates transcription of genes involved in Insulin action, adipocyte differentiation, lipid metabolism and inflammation. It is implicated in numerous diseases including obesity, diabetes, atherosclerosis and cancer. It also activators include prostanoids, fatty acids, thiazolidinediones and N-(2-benzoylphenyl) tyrosine analogues. PPARG may modulate macrophage functions such as proinflammatory activities, and stimulate oxidized low-density lipoprotein (x-LDL) uptake.
• Source: E.coli.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol 0.1M NaCl.
• Purity: > 95% by SDS - PAGE.
• Concentration: 1.0 mg/ml (determined by Bradford assay).
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MADLRALAKH LYDSYIKSFP LTKAKARAIL TGK TTDKSPF VIYDMNSLMM GEDKIKFKHI TPLQEQSKEVAIRIFQGCQF RSVEAVQEIT EYAKSIPGFV NLDLNDQVTL LKYGVHEIIY TMLASLMNKD GVLISEGQGF MTRE FLKSLR KPFGDFMEPKFEFAVKFNAL ELDDSDLAIF IAVIILSGDR PGLLNVKPIE DIQD NLLQAL ELQLKLNHPE SSQLFAKLLQ KMTDLRQIVT EHVQ LLQVIK KTETD MSLHP LLQEIYKDLY.
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Publication:
  A Fragment of Adhesion Molecule L1 Binds to Nuclear Receptors to Regulate Synaptic Plasticity and Motor Coordination (2018)
  Asymmetric Synthesis of the Oxidative Metabolites of Di(2-ethylhexyl) Phthalate and Analysis of Binding with Peroxisome Proliferator-Activated Receptors (2023)
  The functional role of the cell adhesion molecule L1 in mitochondrial metabolism and dynamics and the functional consequences of L1´s interaction with nuclear receptors in the murine central nervous system (2017)

Gene Information

• Gene Name: PPARG peroxisome proliferator-activated receptor gamma [ Homo sapiens ]
• Synonyms: PPARG; peroxisome proliferator-activated receptor gamma; GLM1; CIMT1; NR1C3; PPARG1; PPARG2; PPARgamma; Peroxisome proliferator-activated receptor gamma; Nuclear receptor subfamily 1 group C member 3; peroxisome proliferative activated receptor, gamma; peroxisome proliferative activated receptor gamma; peroxisome proliferator-activated receptor gamma 1; OTTHUMP00000185030; OTTHUMP00000185033; OTTHUMP00000185037
• Gene ID: 5468
• mRNA Refseq: NM_005037
• Protein Refseq: NP_005028
• MIM: 601487
• UniProt ID: P37231
• Chromosome Location: 3p25
• Pathway: Huntington"s disease; PPAR signaling pathway; Pathways in cancer
• Function: arachidonic acid binding; drug binding; metal ion binding; prostaglandin receptor activity; prostaglandin receptor activity; protein heterodimerization activity; retinoid X receptor binding; sequence-specific DNA binding; steroid hormone receptor activity; transcription activator activity; transcription activator binding; transcription activator binding; transcription factor activity; transcription repressor activity; zinc ion binding