||Recombinant human PPARG protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques. MW = 33.0kDa (290aa).
||PPARG, also known as Peroxisome proliferator-activated receptor gamma, is member of the nuclear hormone receptor subfamily of transcription factors. This protein regulates transcription of genes involved in Insulin action, adipocyte differentiation, lipid metabolism and inflammation. It is implicated in numerous diseases including obesity, diabetes, atherosclerosis and cancer. It also activators include prostanoids, fatty acids, thiazolidinediones and N-(2-benzoylphenyl) tyrosine analogues. PPARG may modulate macrophage functions such as proinflammatory activities, and stimulate oxidized low-density lipoprotein (x-LDL) uptake.
||Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol 0.1M NaCl.
||> 95% by SDS - PAGE.
||1.0 mg/ml (determined by Bradford assay).
|Sequences Of Amino Acids:
||MGSSHHHHHH SSGLVPRGSH MADLRALAKH LYDSYIKSFP LTKAKARAIL TGK TTDKSPF VIYDMNSLMM GEDKIKFKHI TPLQEQSKEVAIRIFQGCQF RSVEAVQEIT EYAKSIPGFV NLDLNDQVTL LKYGVHEIIY TMLASLMNKD GVLISEGQGF MTRE FLKSLR KPFGDFMEPKFEFAVKFNAL ELDDSDLAIF IAVIILSGDR PGLLNVKPIE DIQD NLLQAL ELQLKLNHPE SSQLFAKLLQ KMTDLRQIVT EHVQ LLQVIK KTETD MSLHP LLQEIYKDLY.
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.