||Recombinant human CBFB protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography. MW = 23.6 kDa(202aa).
||CBFB, also known as PEBP2B, is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e.g., RUNX1) and osteogenesis (e.g., RUNX2). This protein is a non-DNA binding regulatory subunit and it allosterically enhances DNA binding by alpha subunit.
||Liquid. In 20 mM MES (pH 6.0) containing 0.1 mM PMSF, 10% Glycerol.
||> 85% by SDS - PAGE.
||0.5 mg/ml (determined by Bradford assay).
|Sequences Of Amino Acids:
||MGSSHHHHHH SSGLVPRGSH MPRVVPDQRS KFENEEFFRK LSRECEIKYT GF RDRPHEER QARFQNACRD GRSEIAFVAT GTNLSLQFFPASWQGEQRQT PSREY VD LE R EAGKVYLKAP MILNGVCVIW KGWIDLQRLD GMGCLEFDEE RA QQE DAL AQ QAFEEARRRT REFEDRDRSHREEMEVRVSQ LLAVTGKKTT RP.
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.