Recombinant Human Core-binding Factor, Beta Subunit, His-tagged

• Cat.No.: CBFB-2776H
• Product Overview: Recombinant human CBFB protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography. MW = 23.6 kDa(202aa).

Specification

• Cat. No.: CBFB-2776H
• Description: CBFB, also known as PEBP2B, is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e.g., RUNX1) and osteogenesis (e.g., RUNX2). This protein is a non-DNA binding regulatory subunit and it allosterically enhances DNA binding by alpha subunit.
• Source: E.coli.
• Form: Liquid. In 20 mM MES (pH 6.0) containing 0.1 mM PMSF, 10% Glycerol.
• Purity: > 85% by SDS - PAGE.
• Concentration: 0.5 mg/ml (determined by Bradford assay).
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MPRVVPDQRS KFENEEFFRK LSRECEIKYT GF RDRPHEER QARFQNACRD GRSEIAFVAT GTNLSLQFFPASWQGEQRQT PSREY VD LE R EAGKVYLKAP MILNGVCVIW KGWIDLQRLD GMGCLEFDEE RA QQE DAL AQ QAFEEARRRT REFEDRDRSHREEMEVRVSQ LLAVTGKKTT RP.
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: CBFB core-binding factor, beta subunit [ Homo sapiens ]
• Synonyms: core-binding factor, beta subunit; Polyomavirus enhancer-binding protein 2 beta subunit; PEBP2B; CBFB; core-binding factor subunit beta; CBF-beta; PEA2-beta; SL3-3 enhancer factor 1 beta subunit; SL3-3 enhancer factor 1 subunit beta; SL3/AKV core-binding factor beta subunit; polyomavirus enhancer-binding protein 2 beta subunit; polyomavirus enhancer binding protein 2, beta subunit
• Gene ID: 865
• mRNA Refseq: NM_001755
• Protein Refseq: NP_001746
• MIM: 121360
• UniProt ID: Q13951
• Chromosome Location: 16q22.1
• Function: RNA polymerase II transcription factor activity; protein binding; transcription coactivator activity; transcription factor activity