||Recombinant human DYNLL1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. MW = 12.5 kDa (109aa) confirmed by MALDI-TOF.
||DYNLL1, also known as DLC8 or PIN (protein inhibitor of neuronal nitric oxide synthase), has been identified as a protein that interacts with NOS1 resulting in NOS1 inhibition. Binding of this protein destabilizes NOS1(Neuronal nitric oxide synthase) dimer, a conformation necessary for activity, and it may regulate numerous biologic processes through its effects on nitric oxide synthase activity. DYNLL1 is a ubiquitously expressed protein that exhibits high expression in testis and moderate expression in brain.
|Sequences Of Amino Acids:
||MGSSHHHHHH SSGLVPRGSH MCDRKAVIKN ADMSEEMQQD SVECATQALE KYNIEKDIAA HIKKEFDKKY NPTWHCIVGR NFGSYVTHET KHFIYFYLGQ VAILLFKSG
||> 90% by SDS – PAGE.
||Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.2M NaCl, 1mM DTT, 10% glycerol.
||1 mg/ml (determined by Bradford assay).
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.