Recombinant Human PhosphoHistidine Phosphatase 1, His-tagged
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| Cat. No. : | PHPT1-800H |
| Product Overview : | Recombinant human PHPT1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. MW = 15.9 kDa (145aa) confirmed by MALDI-TOF. |
| Description : | PHPT1 is a 125 amino acid enzyme belonging to the Janus protein family. Existing as a monomer in the cytoplasm, PHPT1 is an EDTA-insensitive phosphohistidine phosphatase. Overexpression of PHPT1 leads to specific phosphohistidine phosphatase activity towards phosphopeptide I, with no activity detected towards phosphotyrosine, phosphothreonine and phosphoserine peptides. |
| Source : | E.coli. |
| Sequences Of Amino Acids : | MGSSHHHHHH SSGLVPRGSH MAVADLALIP DVDIDSDGVF KYVLIRVHSA PRSGAPAAES KEIVRGYKWA EYHADIYDKV SGDMQKQGCD CECLGGGRIS HQSQDKKIHV YGYSMAYGPA QHAISTEKIK AKYPDYEVTW ANDGY |
| Purity : | > 95% by SDS – PAGE. |
| Form : | Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.2M NaCl, 2mM DTT, 10% glycerol. |
| Concentration : | 0.5 mg/ml (determined by Bradford assay). |
| Storage : | Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles. |
| Figure : |
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| Gene Name : | PHPT1 phosphohistidine phosphatase 1 [ Homo sapiens ] |
| Synonyms : | PHPT1; phosphohistidine phosphatase 1; PHP14; CGI-202; HSPC141; bA216L13.10; DKFZp564M173; RP11-216L13.10; 14 kDa phosphohistidine phosphatase; 1700008C22Rik; OTTHUMP00000022620; protein janus-A homolog; sex-regulated protein janus-a; phosphohistidine phosphatase 14kDa; EC 3.1.3.- |
| Gene ID : | 29085 |
| mRNA Refseq : | NM_001135861 |
| Protein Refseq : | NP_001129333 |
| MIM : | 610167 |
| UniProt ID : | Q9NRX4 |
| Chromosome Location : | 9q34.3 |
| Pathway : | Fructose and mannose metabolism; Metabolic pathways; Riboflavin metabolism; Thiamine metabolism |
| Function : | hydrolase activity; phosphohistidine phosphatase activity; phosphoprotein phosphatase activity |
◆ Recombinant Protein
| PHPT1-1482H | Recombinant Human PHPT1 Protein (1-125 aa), His-tagged | +Inquiry |
| PHPT1-753H | Recombinant Human PHPT1 Protein, His-tagged | +Inquiry |
| Phpt1-754M | Recombinant Mouse Phpt1 Protein, His-tagged | +Inquiry |
| PHPT1-5067C | Recombinant Chicken PHPT1 | +Inquiry |
| PHPT1-997Z | Recombinant Zebrafish PHPT1 | +Inquiry |
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◆ Lysates
| PHPT1-3215HCL | Recombinant Human PHPT1 293 Cell Lysate | +Inquiry |
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