Recombinant Human PhosphoHistidine Phosphatase 1, His-tagged

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Recombinant Human PhosphoHistidine Phosphatase 1, His-tagged

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Cat. No. : PHPT1-800H
Product Overview : Recombinant human PHPT1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. MW = 15.9 kDa (145aa) confirmed by MALDI-TOF.
Description : PHPT1 is a 125 amino acid enzyme belonging to the Janus protein family. Existing as a monomer in the cytoplasm, PHPT1 is an EDTA-insensitive phosphohistidine phosphatase. Overexpression of PHPT1 leads to specific phosphohistidine phosphatase activity towards phosphopeptide I, with no activity detected towards phosphotyrosine, phosphothreonine and phosphoserine peptides.
Source : E.coli.
Sequences Of Amino Acids : MGSSHHHHHH SSGLVPRGSH MAVADLALIP DVDIDSDGVF KYVLIRVHSA PRSGAPAAES KEIVRGYKWA EYHADIYDKV SGDMQKQGCD CECLGGGRIS HQSQDKKIHV YGYSMAYGPA QHAISTEKIK AKYPDYEVTW ANDGY
Purity : > 95% by SDS – PAGE.
Form : Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.2M NaCl, 2mM DTT, 10% glycerol.
Concentration : 0.5 mg/ml (determined by Bradford assay).
Storage : Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Figure :

Gene Name : PHPT1 phosphohistidine phosphatase 1 [ Homo sapiens ]
Synonyms : PHPT1; phosphohistidine phosphatase 1; PHP14; CGI-202; HSPC141; bA216L13.10; DKFZp564M173; RP11-216L13.10; 14 kDa phosphohistidine phosphatase; 1700008C22Rik; OTTHUMP00000022620; protein janus-A homolog; sex-regulated protein janus-a; phosphohistidine phosphatase 14kDa; EC 3.1.3.-
Gene ID : 29085
mRNA Refseq : NM_001135861
Protein Refseq : NP_001129333
MIM : 610167
UniProt ID : Q9NRX4
Chromosome Location : 9q34.3
Pathway : Fructose and mannose metabolism; Metabolic pathways; Riboflavin metabolism; Thiamine metabolism
Function : hydrolase activity; phosphohistidine phosphatase activity; phosphoprotein phosphatase activity
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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