Cat. No. :	PHPT1-800H
Product Overview :	Recombinant human PHPT1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. MW = 15.9 kDa (145aa) confirmed by MALDI-TOF.
Description :	PHPT1 is a 125 amino acid enzyme belonging to the Janus protein family. Existing as a monomer in the cytoplasm, PHPT1 is an EDTA-insensitive phosphohistidine phosphatase. Overexpression of PHPT1 leads to specific phosphohistidine phosphatase activity towards phosphopeptide I, with no activity detected towards phosphotyrosine, phosphothreonine and phosphoserine peptides.
Source :	E.coli.
Sequences Of Amino Acids :	MGSSHHHHHH SSGLVPRGSH MAVADLALIP DVDIDSDGVF KYVLIRVHSA PRSGAPAAES KEIVRGYKWA EYHADIYDKV SGDMQKQGCD CECLGGGRIS HQSQDKKIHV YGYSMAYGPA QHAISTEKIK AKYPDYEVTW ANDGY
Purity :	> 95% by SDS – PAGE.
Form :	Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.2M NaCl, 2mM DTT, 10% glycerol.
Concentration :	0.5 mg/ml (determined by Bradford assay).
Storage :	Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Name :	PHPT1 phosphohistidine phosphatase 1 [ Homo sapiens ]
Synonyms :	PHPT1; phosphohistidine phosphatase 1; PHP14; CGI-202; HSPC141; bA216L13.10; DKFZp564M173; RP11-216L13.10; 14 kDa phosphohistidine phosphatase; 1700008C22Rik; OTTHUMP00000022620; protein janus-A homolog; sex-regulated protein janus-a; phosphohistidine phosphatase 14kDa; EC 3.1.3.-
Gene ID :	29085
mRNA Refseq :	NM_001135861
Protein Refseq :	NP_001129333
MIM :	610167
UniProt ID :	Q9NRX4
Chromosome Location :	9q34.3
Pathway :	Fructose and mannose metabolism; Metabolic pathways; Riboflavin metabolism; Thiamine metabolism
Function :	hydrolase activity; phosphohistidine phosphatase activity; phosphoprotein phosphatase activity

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