Recombinant Human PhosphoHistidine Phosphatase 1, His-tagged PHPT1-800H

Recombinant Human PhosphoHistidine Phosphatase 1, His-tagged

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Recombinant Human PhosphoHistidine Phosphatase 1, His-tagged

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Cat. No. : PHPT1-800H
Product Overview : Recombinant human PHPT1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. MW = 15.9 kDa (145aa) confirmed by MALDI-TOF.
Description : PHPT1 is a 125 amino acid enzyme belonging to the Janus protein family. Existing as a monomer in the cytoplasm, PHPT1 is an EDTA-insensitive phosphohistidine phosphatase. Overexpression of PHPT1 leads to specific phosphohistidine phosphatase activity towards phosphopeptide I, with no activity detected towards phosphotyrosine, phosphothreonine and phosphoserine peptides.
Source : E.coli.
Sequences Of Amino Acids : MGSSHHHHHH SSGLVPRGSH MAVADLALIP DVDIDSDGVF KYVLIRVHSA PRSGAPAAES KEIVRGYKWA EYHADIYDKV SGDMQKQGCD CECLGGGRIS HQSQDKKIHV YGYSMAYGPA QHAISTEKIK AKYPDYEVTW ANDGY
Purity : > 95% by SDS – PAGE.
Form : Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.2M NaCl, 2mM DTT, 10% glycerol.
Concentration : 0.5 mg/ml (determined by Bradford assay).
Storage : Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Figure :

Gene Name : PHPT1 phosphohistidine phosphatase 1 [ Homo sapiens ]
Synonyms : PHPT1; phosphohistidine phosphatase 1; PHP14; CGI-202; HSPC141; bA216L13.10; DKFZp564M173; RP11-216L13.10; 14 kDa phosphohistidine phosphatase; 1700008C22Rik; OTTHUMP00000022620; protein janus-A homolog; sex-regulated protein janus-a; phosphohistidine phosphatase 14kDa; EC 3.1.3.-
Gene ID : 29085
mRNA Refseq : NM_001135861
Protein Refseq : NP_001129333
MIM : 610167
UniProt ID : Q9NRX4
Chromosome Location : 9q34.3
Pathway : Fructose and mannose metabolism; Metabolic pathways; Riboflavin metabolism; Thiamine metabolism
Function : hydrolase activity; phosphohistidine phosphatase activity; phosphoprotein phosphatase activity

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