||Recombinant human THYN1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. MW = 27.8 kDa (245aa) confirmed by MALDI-TOF (molecular size on SDS-PAGE will appear higher).
||Thymocyte nuclear protein 1, also known THYN1, is highly conserved among vertebrates and plant species and may be involved in the induction of apoptosis. Also, THYN1 in the thymocytes was mainly localized in the nucleus, as recently demonstrated in lymphoma cells, indicating that the THYN1 resides in the nucleus, irrespective of the cyclic or resting stage of the cell cycle.
|Sequences Of Amino Acids:
||MGSSHHHHHH SSGLVPRGSH MSRPRKRLAG TSGSDKGLSG KRTKTENSGE ALAKVEDSNP QKTSATKNCL KNLSSHWLMK SEPESRLEKG VDVKFSIEDL KAQPKQTTCW DGVRNYQARN FLRAMKLGEE AFFYHSNCKE PGIAGLMKIV KEAYPDHTQF EKNNPHYDPS SKEDNPKWSM VDVQFVRMMK RFIPLAELKS YHQAHKATGG PLKNMVLFTR QRLSIQPLTQ EEFDFVLSLE EKEPS
||> 95% by SDS – PAGE.
||Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 100mM NaCl.
||0.5 mg/ml (determined by Bradford assay).
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.