|Cat. No. :
|Product Overview :
||Recombinant human JAK2 was expressed by baculovirus inSf9 insect cellsusing an N-terminal His tag. MW=42.5kDa.
||JAK2 is a member of intracellular non-receptor tyrosine kinases that transduce cytokine-mediated signals via the JAK-STAT pathway. JAK2 has two near-identical phosphate-transferring domains. One domain exhibits the kinase activity while the other stabilizes the JAK conformational structure. JAK2 is the predominant JAK kinase activated in response to several growth factors and cytokines such as IL-3, GM-CSF and erythropoietin. JAK2 has been found to be constitutively associated with the prolactin receptor and is required for responses to gamma interferon.
||Sf9 insect cells.
|Specific Activity :
||118 pmol/min/μg, Assay conditions: 50 mM HEPES (pH7.5), 10 mM MgCl2, 1 mM EGTA, 200 μM ATP, 0.01% Brij-35, and 2 μM substrate (Tyr 4, Invitrogen) at room temperature for 1 hour. Developer solution was added to reaction and reaction was stopped after 1h of incubation at RT.
||Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
|Formulated In :
||25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 10% glycerol, and 1 mM DTT.
||>6 months at –80°C.