||Recombinant human MUSK was expressed by baculovirus inSf9 insect cellsusing an N-terminal His tag. MW=38.5kDa.
||MUSK is a receptor tyrosine kinase necessary for neuromuscular junction formation. MUSK gene expression is highly regulated during neuromuscular junction and it is involved in intercellular communication present on the surface of cells that are activated by specific protein ligands. MUSK members play a key role in growth and differentiation of those cell types. Agrin signals through MUSK to cluster acetylcholine receptors on the postsynaptic membrane of the neuromuscular junction. DOK7, a MUSK-interacting cytoplasmic protein, is essential for MUSK activation in cultured myotubes. MUSK also plays a critical role in the development of normal blood vessels.
||Sf9 insect cells.
||6.9 pmole/min/mg Assay conditions: Add 50 µl reaction mix (MUSK diluted in 1x kinase buffer supplemented with 0.2 mM ATP) to wells coated with substrate. Incubate for 1h. Add Europeum-labeled anti p-Tyr antibody and incubate for 1h. Time resolved fluorescence measured at room temperature atλexc=330±40 nm and λem=620±10 nm.
||Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
||25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol, and 1 mM DTT.
||>12 months at –80°C.