"NEK6" Related Products


Recombinant Human NEK6, His-tagged

Cat. No.: NEK6-559H
Product Overview: Recombinant human NEK6 was expressed bybaculovirus in Sf9 insect cellsusing an N-terminal His tag. MW=40.1kDa.
Description: NEK6 is a serine/threonine kinase that is a member of the Nek family of protein kinases that share an amino-terminal catalytic domain related to NIMA family. Nek6 is a nuclear and cytoplasmic kinase that is required for mitotic progression of human cells. Nek6 is phosphorylated and activated during M phase of the cell cycle. Inhibition of Nek6 function by either overexpression of an inactive Nek6 mutant or elimination of endogenous Nek6 by siRNA arrests cells in M phase and triggers apoptosis.
Source: Baculovirus infected Sf9 cells.
Purity: >90 % by SDS-Page.
Specific Activity: 70 U/mg. One unit is defined as the amount of enzyme that will phosphorylate 1 nmol NEK6 substrate (FLAKSFGSPNRAYKK) per minute at pH 7.4 and 30°C. Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 uM Naorthovanadate, 0.1 mM ATP, 4 uM NEK6 substrate, and 0.4 ug/ml NEK6.
Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Formulated In: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, and 1 mM DTT.
Stability: >6 months at –80°C.
Gene Name: NEK6 NIMA (never in mitosis gene a)-related kinase 6 [ Homo sapiens ]
Synonyms: NEK6; NIMA (never in mitosis gene a)-related kinase 6; SID6-1512; NIMA-related kinase 6; OTTHUMP00000022097; putative serine-threonine protein kinase; EC
Gene ID: 10783
mRNA Refseq: NM_001145001
Protein Refseq: NP_001138473
MIM: 604043
UniProt ID: P51955
Chromosome Location: 9q33.3-q34.11
Function: ATP binding; magnesium ion binding; nucleotide binding; protein phosphatase binding; protein serine/threonine kinase activity; transferase activity; protein serine/threonine kinase activity; signal transducer activity

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