||Recombinant human NEK6 was expressed bybaculovirus in Sf9 insect cellsusing an N-terminal His tag. MW=40.1kDa.
||NEK6 is a serine/threonine kinase that is a member of the Nek family of protein kinases that share an amino-terminal catalytic domain related to NIMA family. Nek6 is a nuclear and cytoplasmic kinase that is required for mitotic progression of human cells. Nek6 is phosphorylated and activated during M phase of the cell cycle. Inhibition of Nek6 function by either overexpression of an inactive Nek6 mutant or elimination of endogenous Nek6 by siRNA arrests cells in M phase and triggers apoptosis.
||Baculovirus infected Sf9 cells.
||>90 % by SDS-Page.
||70 U/mg. One unit is defined as the amount of enzyme that will phosphorylate 1 nmol NEK6 substrate (FLAKSFGSPNRAYKK) per minute at pH 7.4 and 30°C. Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 uM Naorthovanadate, 0.1 mM ATP, 4 uM NEK6 substrate, and 0.4 ug/ml NEK6.
||Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
||25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, and 1 mM DTT.
||>6 months at –80°C.