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Recombinant Human Synaptotagmin I, His-tagged

Cat. No.: SYT1-5131H
Product Overview: Recombinant humanSYT1 protein, fused to His-tag at C-terminus, was expressed in E. coli andpurified by using conventional chromatography techniques.
Description: SYT1(Synaptotagmin-1) belongs to the synaptotagmin family and contains 2 C2domains. The synaptotagmins are integral membrane proteins of synapticvesicles thought to serve as Ca(2+) sensors in the process ofvesicular trafficking and exocytosis. SYT1 is the master switch responsiblefor allowing the human brain to release neurotransmitters.
Concentration: 0.25 mg/ml
Source: Human
Host: E.coli
Form: Suppliedas a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 2mM DTT, 20% glyceroland 100mM NaCl.
Purity: > 85% by SDS -PAGE
Sequence: 136-382 amino acids:MEPKEEEKLG KLQYSLDYDF QNNQLLVGII QAAELPALDM GGTSDPYVKV FLLPDKKKKF ETKVHRKTLNPVFNEQFTFK VPYSELGGKT LVMAVYDFDR FSKHDIIGEF KVPMNTVDFG HVTEEWRDLQ SAEKEEQEKLGDICFSLRYV PTAGKLTVVI LEAKNLKKMD VGGLSDPYVK IHLMQNGKRL KKKKTTIKKN TLNPYYNESFSF
Molecular Mass: 29.5 kDa(256aa), confirmed by MALDI-TOF.
Applications: SDS-PAGE
Storage: Storeat 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg Cor - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
OfficialSymbol: SYT1
Gene Name: SYT1synaptotagmin I [ Homo sapiens ]
Synonyms: SYT1; synaptotagmin I; P65; SYT; SVP65; DKFZp781D2042; synaptotagmin-1sytI; synaptotagmin 1; Synaptotagmin-1; Synaptotagmin I; SytI; OTTHUMP00000166063;OTTHUMP00000166064
Gene ID: 6857
mRNA Refseq: NM_001135805
Protein Refseq: NP_001129277
MIM: 185605
UniProt ID: P21579
Chromosome Location: 12cen-q21
Pathway: AcetylcholineNeurotransmitter Release Cycle; Botulinum neurotoxicity; DopamineNeurotransmitter Release Cycle; Effects of Botulinum toxin; GABA synthesis,release; Glutamate Neurotransmitter Release Cycle; Neurotransmitter ReleaseCycle; Norepinephrine Neurotransmitter Release Cycle; SerotoninNeurotransmitter Release Cycle; Synaptic Transmission; Synaptic vesicle cycle;Translocation of BoNT Light chain; Transmission across Chemical Synapses
Function: 1-phosphatidylinositolbinding; calcium ion binding; calcium-dependentprotein binding; calmodulin binding; identical protein binding; low-densitylipoprotein receptor binding; phospholipid binding; protein binding; syntaxin-1 binding; transporter activity

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