Recombinant Human PTPRB, GST-tagged
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Cat. No. : | PTPRB-1859H |
Product Overview : | Recombinant human PTPRB protein(1675-1996) with N-terminal GST tag was expressed in anE.coliexpression system, MW= 63.7 kDa. |
Description : | Receptor-type tyrosine-protein phosphatase beta is an enzyme that in humans is encoded by the PTPRB gene. The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. |
Source : | E.coli. |
Formulation : | 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, 10 mM glutathione. |
Purity : | > 95% by SDS-PAGE. |
Specific Activity : | 40 U/ug. One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C. Assay buffer: 50 mM HEPES, pH 7.4, 2 mM EDTA, 3 mM DTT, 100 mM NaCl, 50 mM pNPP. |
Application : | Useful for the study of enzyme kinetics, regulation, and to dephosphorylate target substrates. |
Storage : | >6 months at –80°C. |
Pathways : | Adherens junction |
PDB rendering based on 2ahs : |
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Gene Name : | PTPRB protein tyrosine phosphatase, receptor type, B [ Homo sapiens ] |
Synonyms : | protein tyrosine phosphatase, receptor type, B; PTPB; HPTPB; VEPTP; FLJ44133; MGC59935; HPTP-BETA; MGC142023; R-PTP-BETA; DKFZp686E2262; DKFZp686H15164; PTPRB; receptor-type tyrosine-protein phosphatase beta;VE-PTP; EC 3.1.3.48; protein-tyrosine phosphatase beta; vascular endothelial protein tyrosine phosphatase; protein tyrosine phosphatase, receptor type, beta polypeptide;R-PTP-beta;Protein-tyrosine phosphatase beta |
Gene ID : | 5787 |
mRNA Refseq : | NM_001109754 |
Protein Refseq : | NP_001103224 |
MIM : | 176882 |
UniProt ID : | P23467 |
Chromosome Location : | 12q15-q21 |
Function : | hydrolase activity; protein binding; transmembrane receptor protein tyrosine phosphatase activity |
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