||Recombinant human PTPRB protein(1675-1996) with N-terminal GST tag was expressed in anE.coliexpression system, MW= 63.7 kDa.
||Receptor-type tyrosine-protein phosphatase beta is an enzyme that in humans is encoded by the PTPRB gene. The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation.
||25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, 10 mM glutathione.
||> 95% by SDS-PAGE.
||40 U/ug. One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C. Assay buffer: 50 mM HEPES, pH 7.4, 2 mM EDTA, 3 mM DTT, 100 mM NaCl, 50 mM pNPP.
||Useful for the study of enzyme kinetics, regulation, and to dephosphorylate target substrates.
||>6 months at –80°C.