Active Recombinant Human Secreted Phosphoprotein 1

• Cat.No.: SPP1-5130H
• Product Overview: Recombinant human SPP1expressed by 293cells is a 60-65 kDa Osteopontin protein.

Specification

• Cat. No.: SPP1-5130H
• Description: SPP1 is a secreted glycoprotein thatfunctions as a ligand to alphavbeta3 integrin and possibly other receptors.It binds tightly to hydroxyapatite and can act as a structural component ofthe extracellular mineralized matrix. SPP1 is initially secreted as a 298amino acid protein, which is subject to multiple post-translationalmodifications including glycosylation, phosphorylation, and specificproteolytic cleavages into various smaller molecular weight fragments.
• Source: Human
• Host: 293 cells
• Form: Supplied as alyophilized powder. Reconstitute in water to a concentration of 0.1-1.0mg/ml.
• Purity: > 97% pure by SDS PAGE and HPLC
• Sequence: Region of Osteopontin proteincorresponding to amino acids IPVKQADSGS SEEKQLYNKY PDAVATWLNP DPSQKQNLLAPQNAVSSEET NDFKQETLPS KSNESHDHMD DMDDEDDDDH VDSQDSIDSN DSDDVDDTDD SHQSDESHHSDESDELVTDF PTDLPATEVF TPVVPTVDTY DGRGDSVVYG LRSKSKKFRR PDIQYPDATD EDITSHMES
• Molecular Mass: 60-65 kDa
• Endotoxin Levels: Endotoxin level is less than 0.1 ngper ug (1 EU/ug).
• Bioactivity: Determined by its ability tochemoattract human monocytes using a concentration range of 10.0-100.0 ng/ml.
• Applications: Numerous applications are possiblewith this product and should be tested by the end user under their ownlaboratory conditions.
• Storage: Store at 4 deg Cuntil reconstitution. Following reconstitution aliquot and freeze at -20 degC for long term storage. Avoid repeated freeze/thaw cycles.
• OfficialSymbol: SPP1

Gene Information

• Gene Name: SPP1 secreted phosphoprotein 1 [ Homo sapiens]
• Synonyms: SPP1; secretedphosphoprotein 1; OPN; BNSP; BSPI; ETA-1; MGC110940; osteopontin; SPP-1; Uropontin; Nephropontin; osteopontin-C;osteopontin-D; OTTHUMP00000161173; OTTHUMP00000161174; OTTHUMP00000218555; OTTHUMP00000218556;SPP1/CALPHA1 fusion; bone sialoprotein 1; bone sialoprotein I; urinary stoneprotein; early T-lymphocyte activation 1; secreted phosphoprotein-1(osteopontin, bone sialoprotein); osteopontin/immunoglobulin alpha 1 heavychain constant region fusion protein; secreted phosphoprotein 1 (osteopontin,bone sialoprotein I, early T-lymphocyte activation 1)
• Gene ID: 6696
• mRNA Refseq: NM_000582
• Protein Refseq: NP_000573
• MIM: 166490
• UniProt ID: P10451
• Chromosome Location: 4q22.1
• Pathway: Direct p53 effectors;ECM-receptor interaction; Endochondral Ossification; FGF signaling pathway;Focal Adhesion; Integrin cell surface interactions; Osteoclast Signaling;Osteopontin Signaling; Osteopontin-mediated events; Signaling by PDGF; TGFBeta Signaling Pathway; Toll-like receptor signaling pathway
• Function: cytokineactivity; extracellular matrix binding