||Recombinant Human Ret (a.a. 658-end) with Nterminal GST tag expressed in anBaculovirus infected Sf9 cellexpression system. MW= 77.5 kDa.
||The RET proto-oncogene encodes a receptor tyrosine kinase for members of the glial cell line-derived neurotrophic factor family of extracellular signalling molecules. RET loss of function mutations are associated with the development of Hirschsprung"s disease, while gain of function mutations are associated with the development of various types of human cancer, including medullary thyroid carcinoma, multiple endocrine neoplasias type 2A and 2B, phaeochromocytoma and parathyroid hyperplasia.
||Baculovirus infected Sf9 cells.
||Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
||40 U/ug. One unit is defined as the amount of enzyme that will transfer 1 pmol phosphate to Tyr substrate per minute at pH 7.4 and 30°C. Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 uM Na-orthovanadate, 0.1 mM ATP, 30 ug/ml Poly (Glu:Tyr)4:1 substrate, and 2 ug/ml recombinant cMet.
||>80% by SDS-PAGE.
||25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, 10 mM reduced glutathione, and 3 mM DTT.
||>6 months at -80°C.