Recombinant Human Interleukin 7, HQ-tagged


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Recombinant Human Interleukin 7, HQ-tagged

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Cat. No. : IL7-5333H
Product Overview : Recombinanthuman IL7 contains 153 amino acids and a 16 aa HQ tag. Total length is 169aa.
Description : IL7 stimulates thedifferentiation of multipotent hematopoietic stem cells into lymphoidprogenitor cells (as opposed to myeloid progenitor cells wheredifferentiation is stimulated by IL-3).It also stimulates proliferation ofall cells in the lymphoid lineage (B cells, T cells and NK cells). It is importantfor proliferation during certain stages of B-cell maturation, T and NK cellsurvival, development and homeostasis.
Source : Hordeum vulgare(barley grain)
Species : Human
Form : Lyophilized from a0.2 µm sterile filtered solution of PBS, pH 7.2.
Molecular Weight : Predicted MW = 19.7kDa including HQ tag. As a result of glycosylation, the recombinant proteinmigrates as two bands with an apparent MW = 19 and 21 kDa in SDS-PAGE.
Purity : >95% by SDS-PAGE.Purified product carries no pyrogenic or pro-inflammatory contaminants, asassayed with monocyte activation test using custom human Multiplex CytokineAssay measuring IL-6, TNF-alpha and IL-1beta induction.
Endotoxin Level : < 0.005 ng perµg of product (0.05 EU/µg) as measured by turbidimetric kinetic assay.
Biological Activity : The bioactivity of human IL7 was determined bydose-dependent cell proliferation assay on human bone marrow mononuclearcells, measuring intracellular ATP concentration with standardizedluminometry. EC50 for a typical batch lies in the range of 48-72 ng/ml.
Reconstitution : Centrifuge vial prior to opening. First add steriledistilled water to the vial to fully solubilize the protein to aconcentration not less than 100 µg/ml. After complete solubilization of theprotein, it can be further diluted to other aqueous solutions.
Storage : The lyophilizedprotein, though stable at room temperature for few weeks, is best stored at-20°C to -80°C. Reconstituted protein should be used immediately or stored inworking aliquots at -20°C to -80°C. Avoid repeated freeze-thaw cycles.
OfficialSymbol : IL7
Rendering based onPDB 1IL7. :

Gene Name : IL7 interleukin 7 [ Homosapiens ]
Synonyms : IL7; interleukin 7;IL-7; interleukin-7; OTTHUMP00000227044; OTTHUMP00000227046
Gene ID : 3574
mRNA Refseq : NM_000880
Protein Refseq : NP_000871
MIM : 146660
UniProt ID : P13232
Chromosome Location : 8q12-q13
Pathway : Cytokine-cytokinereceptor interaction; Hematopoietic cell lineage; Jak-STAT signaling pathway
Function : cytokine activity;cytokine receptor binding; growth factor activity; interleukin-7 receptorbinding; protein binding
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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