Recombinant Dog Interleukin 3 (colony-stimulating factor, multiple)

• Cat.No.: IL3-362D
• Product Overview: Recombinant DogInterleukin-3 (IL-3) is a single non-glycosylated polypeptide chaincontaining 120 amino acids.

Specification

• Cat. No.: IL3-362D
• Description: IL-3 is a hematopoieticgrowth factor that promotes the survival, differentiation and proliferationof committed progenitor cells of the megakaryocyte, granulocyte-macrophage,erythroid, eosinophil, basophil and mast cell lineages. Produced by T cells,mast cells and eosinophils, IL-3 enhances thrombopoieses, phagocytosis, andantibody-mediated cellular cytotoxicity. Its ability to activate monocytessuggests that IL-3 may have additional immunoregulatory roles. Many oftheIL-3 activities depend upon costimulation with other cytokines. IL-3 is aspecies-specific, variably glycosylated cytokine.
• Source: E. coli
• Species: Human
• Formulation: Lyophilized from a0.2 um filtered concentrated solution in PBS, pH 7.4.
• MolecularWeight: 14.0 kDa
• Purity: >97% by SDS-PAGEand HPLC analyses
• Endotoxin Level: <0.1 ng/ug ofprotein
• Biological Activity: The ED50 asdetermined by the dose-dependent stimulation of the proliferation of humanTF-1 cells is less than 0.2 ng/ml, corresponding to a specific activity of5.0X10⁶ IU/mg.
• Reconstitution: Centrifuge vialpriorto opening. Reconstitute in sterile distilled water or aqueous buffercontaining 0.1%BSA to a concentration of 0.1-1.0 mg/ml. Stock solutionsshould be divided into working aliquots and stored at -800C. Furtherdilutions should be made in appropriate buffered solutions.
• Amino Acid Sequence: RPFSTDLPKQFTMINEIMEMLNKSPSPSE EPLDSNEKET LLEDTLLRPN LDVFLNASSK FHKNGLLIWN NLKEFLPLLP TPTPRGEPISIMENNWGDFQ RKLKKYLEALDNFLNFKNKP
• Storage: The lyophilizedprotein is stable at 2-4°C, but should be kept desiccated at -20°C for longterm storage. After reconstitution, the protein is stable for 1 week at2-4°C. For long term storage, aliquot and freeze at -80°C. Avoid repeatedfreeze-thaw cycles.
• OfficialSymbol: IL3

Gene Information

• Gene Name: IL3 interleukin 3(colony-stimulating factor, multiple) [ Canis lupus familiaris ]
• Synonyms: IL3; interleukin 3(colony-stimulating factor, multiple); IL-3; MCGF; interleukin-3; mast cellgrowth factor; P-cell-stimulating factor; hematopoietic growth factor;multipotential colony-stimulating factor
• Gene ID: 481497
• mRNA Refseq: NM_001013835
• Protein Refseq: NP_001013857
• UniProt ID: Q9BDX4
• Pathway: Apoptosis; Asthma;Cytokine Signaling in Immune system; Cytokine-cytokine receptor interaction;Fc epsilon RI signaling pathway; Hematopoietic cell lineage; Immune System;Interleukin receptor SHC signaling; Interleukin-2 signaling; Interleukin-3, 5and GM-CSF signaling; Jak-STAT signaling pathway; Signaling by Interleukins