Recombinant Human Interleukin 6 (interferon, beta 2), HQ-tagged

Cat. No.: IL6-5309H
Product Overview: Recombinant human IL-6is a 20.9 kDa protein containing 184 amino acid residues. Human IL-6 isactive on murine cells but murine IL-6 is inactive on human cells.
Description: IL-6 is amultifunctional cytokine that has an important role in host defense byregulating immune and inflammatory responses. It is expressed by a variety ofnormal and transformed cells including T cells, B cells,monocytes/macrophages, fibroblasts and various tumor cells. IL-6 has diversebiological functions, it stimulates B-cell differentiation and antibodyproduction, synergizes with IL-3 in megakaryocyte development and plateletproduction, induces expression of hepatic acute-phase proteins, and regulatesbone metabolism.
Source: Hordeum vulgare(barley grain)
Species: Human
Form: Sterile filteredthrough a 0.2 um filter. Lyophilized from 50 mM MES pH 6.5, 500 mM NaCl.
Molecular Weight: 23.9 kDa
Purity: Greater than 95% bySDS-PAGE gel analysis. Purified product carries no pyrogenic orpro-inflammatory contaminants, as assayed with monocyte activation test usingHuman 10-plex Cytokine Assay measuring IL-6, TNF-alpha and IL-1 betainduction.
Endotoxin Level: Endotoxin level isless than 0.005ng per ug of product (0.05EU/ug) as measured by turbidimetrickinetic assay.
Biological Activity: Bioactivity isassayed by measuring the dose dependent effect of human IL-6 on proliferationof TF-1 cells. The EDSO for this batch of recombinant human IL-6 is 0.20ng/ml, corresponding to 5.0 x 106 U/mg specific activity. Optimalconcentration should be determined for specific applications and cell lines.
Reconstitution: Centrifuge vialprior to opening. First add sterile distilled water to the vial to fullysolubilize the protein to a concentration not less than 100 ug/ml. Aftercomplete solubilization of the protein, it can be further diluted to otheraqueous solutions.
Storage: Store lyophilizedprotein at -20°C to -80°C. Reconstituted protein is stable for 1 week at2-4°C. For long term storage, aliquot and store at -20°C to -80°C with acarrier protein (0.1%HSA or BSA) as a stabilizer. Please note that theaddition of any carrier protein into this product may produce unwantedendotoxin. This depends upon the particular application employed. Avoidrepeated freeze-thaw cycles.
OfficialSymbol: IL6
Gene Name: IL6 interleukin 6 (interferon,beta 2) [ Homo sapiens ]
Synonyms: IL6; interleukin 6(interferon, beta 2); HGF; HSF; BSF2; IL-6; IFNB2; interleukin-6; CDF; BSF-2;IFN-beta-2; interferon beta-2; interleukin BSF-2; OTTHUMP00000158544; OTTHUMP00000198486;OTTHUMP00000198489; OTTHUMP00000198490; hybridoma growth factor; CTLdifferentiation factor; B cell stimulatory factor-2; B-cell stimulatoryfactor 2; B-cell differentiation factor
Gene ID: 3569
mRNA Refseq: NM_000600
Protein Refseq: NP_000591
MIM: 147620
UniProt ID: P05231
Chromosome Location: 7p21-p15
Pathway: ATF-2 transcriptionfactor network; Adipogenesis; African trypanosomiasis; Amoebiasis; AndrogenReceptor Signaling Pathway; Cytokine Signaling in Immune system; Cytokinesand Inflammatory Response; Cytosolic DNA-sensing pathway; Graft-versus-hostdisease; HTLV-I infection; Hematopoietic cell lineage; Herpes simplexinfection; IL-6 Signaling Pathway; Immune System; Influenza A
Function: cytokine activity;growth factor activity; interleukin-6 receptor binding; contributes_tointerleukin-6 receptor binding

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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