Native Human Glutamic-Pyruvate Transaminase (alanine aminotransferase)

• Cat.No.: GPT-5344H
• Product Overview: Native Human GPT isprepared from Human Liver.

Specification

• Cat. No.: GPT-5344H
• Description: GPTis an enzyme that catalyzes the reversible transfer of an amino group fromalanine to α-ketoglutarate. Human GPT is found in serum and in various bodilytissues but is most commonly associated with the liver.
• Form: Ammonia Sulphatesuspension.
• Source: Human Liver
• Molecular Weight: 100 kDa
• Specific Activity: >10 U/mg protein
• Endotoxin Level: < 0.1 ng/ug ofprotein
• Biological Activity: > 50 U/ml at37°C. One unit will catalyze the transamination of one micromole of L-alanineto α-ketoglutarate, forming L-glutamate and pyruvate per minute at 37°C andpH 7.8. Measured at 340 nm as one equimolar amount of NAD produced by acoupled reaction.
• Storage: Store at 2-4°C.Stable for 1 year.
• Pathways: Alanine, aspartate andglutamate metabolism; Biosynthesis of alkaloids derived from ornithine,lysine and nicotinic acid; Carbon fixation in photosynthetic organisms;Metabolic pathways; Metabolism of amino acids

Gene Information

• Gene Name: GPT glutamic-pyruvatetransaminase (alanine aminotransferase) [ Homo sapiens ]
• Official Symbol: GPT
• Synonyms: GPT; glutamic-pyruvatetransaminase (alanine aminotransferase); AAT1; ALT1; GPT1; Alanineaminotransferase 1; Glutamic--pyruvic transaminase 1; Glutamate pyruvatetransaminase 1; EC 2.6.1.2; glutamic pyruvate transaminase
• Gene ID: 2875
• mRNA Refseq: NM_005309
• Protein Refseq: NP_005300
• MIM: 138200
• UniProt ID: P24298
• Chromosome Location: 8q24.3
• Function: 1-aminocyclopropane-1-carboxylatesynthase activity; L-alanine:2-oxoglutarate aminotransferase activity;pyridoxal phosphate binding; transaminase activity