Cat. No. : |
GIF-5359H |
Product Overview : |
Recombinant Human GIFproduced in baculovirus is a glycosylated, polypeptide chain containinghaving a molecular mass of 55,000 Dalton. rHu GIF is fused to ahexa-histidine at the C-terminus and purified by proprietary chromatographictechniques for removal of bound Vitamin B-12. |
Description : |
GIF is a memberof the cobalamin transport protein family. It encodes a glycoprotein secretedby parietal cells of the gastric mucosa and is required for adequateabsorption of vitamin B12 in the terminal ileum. |
Source : |
Sf9 Insect Cells |
Species : |
Human |
Form : |
The protein solution (0.7 mg/ml)contains 20 mM HEPES pH8, 100 mM NaCl, 20% Glycerol. |
Purity : |
Greater than 95.0% asdetermined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
Endotoxin Level : |
Less than 0.1 ng/ug(IEU/ug) of recombinant Human GIF. |
Biological Activity : |
Standard ELISA test(checker-board analysis of positive/negative sera panels), immunodot withanti-IF autoantibody positive patient sera. Can be used for solid ELISA andfluid phase diagnostic assays, 0.25-0.65µg/ml. Suitable for biotinylation andiodination. |
Storage : |
GIF althoughstable at 10°C for 1 week, should be storeddesiccated below -18°C. For long termstorage it is recommended to add a carrier protein (0.1% HSA or BSA). Pleaseprevent freeze-thaw cycles. |
OfficialSymbol : |
GIF |