Recombinant Human Gastric Intrinsic Factor (vitamin B synthesis)


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Recombinant Human Gastric Intrinsic Factor (vitamin B synthesis)

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Cat. No. : GIF-5359H
Product Overview : Recombinant Human GIFproduced in baculovirus is a glycosylated, polypeptide chain containinghaving a molecular mass of 55,000 Dalton. rHu GIF is fused to ahexa-histidine at the C-terminus and purified by proprietary chromatographictechniques for removal of bound Vitamin B-12.
Description : GIF is a memberof the cobalamin transport protein family. It encodes a glycoprotein secretedby parietal cells of the gastric mucosa and is required for adequateabsorption of vitamin B12 in the terminal ileum.
Source : Sf9 Insect Cells
Species : Human
Form : The protein solution (0.7 mg/ml)contains 20 mM HEPES pH8, 100 mM NaCl, 20% Glycerol.
Purity : Greater than 95.0% asdetermined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Endotoxin Level : Less than 0.1 ng/ug(IEU/ug) of recombinant Human GIF.
Biological Activity : Standard ELISA test(checker-board analysis of positive/negative sera panels), immunodot withanti-IF autoantibody positive patient sera. Can be used for solid ELISA andfluid phase diagnostic assays, 0.25-0.65µg/ml. Suitable for biotinylation andiodination.
Storage : GIF althoughstable at 10°C for 1 week, should be storeddesiccated below -18°C. For long termstorage it is recommended to add a carrier protein (0.1% HSA or BSA). Pleaseprevent freeze-thaw cycles.
OfficialSymbol : GIF
Gene Name : GIF gastric intrinsic factor(vitamin B synthesis) [ Homo sapiens ]
Synonyms : GIF; gastric intrinsic factor(vitamin B synthesis); IF; INF; IFMH; TCN3; Gastric intrinsic factor;Intrinsic factor
Gene ID : 2694
mRNA Refseq : NM_005142
Protein Refseq : NP_005133
MIM : 609342
UniProt ID : P27352
Chromosome Location : 11
Pathway : Vitamin digestionand absorption
Function : cobalamin binding
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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