"GIF" Related Products

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Recombinant Human Gastric Intrinsic Factor (vitamin B synthesis)

Cat. No.: GIF-5359H
Product Overview: Recombinant Human GIFproduced in baculovirus is a glycosylated, polypeptide chain containinghaving a molecular mass of 55,000 Dalton. rHu GIF is fused to ahexa-histidine at the C-terminus and purified by proprietary chromatographictechniques for removal of bound Vitamin B-12.
Description: GIF is a memberof the cobalamin transport protein family. It encodes a glycoprotein secretedby parietal cells of the gastric mucosa and is required for adequateabsorption of vitamin B12 in the terminal ileum.
Source: Sf9 Insect Cells
Species: Human
Form: The protein solution (0.7 mg/ml)contains 20 mM HEPES pH8, 100 mM NaCl, 20% Glycerol.
Purity: Greater than 95.0% asdetermined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Endotoxin Level: Less than 0.1 ng/ug(IEU/ug) of recombinant Human GIF.
Biological Activity: Standard ELISA test(checker-board analysis of positive/negative sera panels), immunodot withanti-IF autoantibody positive patient sera. Can be used for solid ELISA andfluid phase diagnostic assays, 0.25-0.65µg/ml. Suitable for biotinylation andiodination.
Storage: GIF althoughstable at 10°C for 1 week, should be storeddesiccated below -18°C. For long termstorage it is recommended to add a carrier protein (0.1% HSA or BSA). Pleaseprevent freeze-thaw cycles.
OfficialSymbol: GIF
Gene Name: GIF gastric intrinsic factor(vitamin B synthesis) [ Homo sapiens ]
Synonyms: GIF; gastric intrinsic factor(vitamin B synthesis); IF; INF; IFMH; TCN3; Gastric intrinsic factor;Intrinsic factor
Gene ID: 2694
mRNA Refseq: NM_005142
Protein Refseq: NP_005133
MIM: 609342
UniProt ID: P27352
Chromosome Location: 11
Pathway: Vitamin digestionand absorption
Function: cobalamin binding

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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