Cat. No.:	GIF-5359H
Product Overview:	Recombinant Human GIFproduced in baculovirus is a glycosylated, polypeptide chain containinghaving a molecular mass of 55,000 Dalton. rHu GIF is fused to ahexa-histidine at the C-terminus and purified by proprietary chromatographictechniques for removal of bound Vitamin B-12.
Description:	GIF is a memberof the cobalamin transport protein family. It encodes a glycoprotein secretedby parietal cells of the gastric mucosa and is required for adequateabsorption of vitamin B12 in the terminal ileum.
Source:	Sf9 Insect Cells
Species:	Human
Form:	The protein solution (0.7 mg/ml)contains 20 mM HEPES pH8, 100 mM NaCl, 20% Glycerol.
Purity:	Greater than 95.0% asdetermined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Endotoxin Level:	Less than 0.1 ng/ug(IEU/ug) of recombinant Human GIF.
Biological Activity:	Standard ELISA test(checker-board analysis of positive/negative sera panels), immunodot withanti-IF autoantibody positive patient sera. Can be used for solid ELISA andfluid phase diagnostic assays, 0.25-0.65µg/ml. Suitable for biotinylation andiodination.
Storage:	GIF althoughstable at 10°C for 1 week, should be storeddesiccated below -18°C. For long termstorage it is recommended to add a carrier protein (0.1% HSA or BSA). Pleaseprevent freeze-thaw cycles.
OfficialSymbol:	GIF

Gene Name:	GIF gastric intrinsic factor(vitamin B synthesis) [ Homo sapiens ]
Synonyms:	GIF; gastric intrinsic factor(vitamin B synthesis); IF; INF; IFMH; TCN3; Gastric intrinsic factor;Intrinsic factor
Gene ID:	2694
mRNA Refseq:	NM_005142
Protein Refseq:	NP_005133
MIM:	609342
UniProt ID:	P27352
Chromosome Location:	11
Pathway:	Vitamin digestionand absorption
Function:	cobalamin binding

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