Recombinant Human Gastric Intrinsic Factor (vitamin B synthesis)
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| Cat. No. : | GIF-5359H |
| Product Overview : | Recombinant Human GIFproduced in baculovirus is a glycosylated, polypeptide chain containinghaving a molecular mass of 55,000 Dalton. rHu GIF is fused to ahexa-histidine at the C-terminus and purified by proprietary chromatographictechniques for removal of bound Vitamin B-12. |
| Description : | GIF is a memberof the cobalamin transport protein family. It encodes a glycoprotein secretedby parietal cells of the gastric mucosa and is required for adequateabsorption of vitamin B12 in the terminal ileum. |
| Source : | Sf9 Insect Cells |
| Species : | Human |
| Form : | The protein solution (0.7 mg/ml)contains 20 mM HEPES pH8, 100 mM NaCl, 20% Glycerol. |
| Purity : | Greater than 95.0% asdetermined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
| Endotoxin Level : | Less than 0.1 ng/ug(IEU/ug) of recombinant Human GIF. |
| Biological Activity : | Standard ELISA test(checker-board analysis of positive/negative sera panels), immunodot withanti-IF autoantibody positive patient sera. Can be used for solid ELISA andfluid phase diagnostic assays, 0.25-0.65µg/ml. Suitable for biotinylation andiodination. |
| Storage : | GIF althoughstable at 10°C for 1 week, should be storeddesiccated below -18°C. For long termstorage it is recommended to add a carrier protein (0.1% HSA or BSA). Pleaseprevent freeze-thaw cycles. |
| OfficialSymbol : | GIF |
| Gene Name : | GIF gastric intrinsic factor(vitamin B synthesis) [ Homo sapiens ] |
| Synonyms : | GIF; gastric intrinsic factor(vitamin B synthesis); IF; INF; IFMH; TCN3; Gastric intrinsic factor;Intrinsic factor |
| Gene ID : | 2694 |
| mRNA Refseq : | NM_005142 |
| Protein Refseq : | NP_005133 |
| MIM : | 609342 |
| UniProt ID : | P27352 |
| Chromosome Location : | 11 |
| Pathway : | Vitamin digestionand absorption |
| Function : | cobalamin binding |
◆ Recombinant Protein
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◆ Lysates
| GIF-1247HCL | Recombinant Human GIF cell lysate | +Inquiry |
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