Recombinant Human Gastric Intrinsic Factor (vitamin B synthesis)

• Cat.No.: GIF-5359H
• Product Overview: Recombinant Human GIFproduced in baculovirus is a glycosylated, polypeptide chain containinghaving a molecular mass of 55,000 Dalton. rHu GIF is fused to ahexa-histidine at the C-terminus and purified by proprietary chromatographictechniques for removal of bound Vitamin B-12.

Specification

• Cat. No.: GIF-5359H
• Description: GIF is a memberof the cobalamin transport protein family. It encodes a glycoprotein secretedby parietal cells of the gastric mucosa and is required for adequateabsorption of vitamin B12 in the terminal ileum.
• Source: Sf9 Insect Cells
• Species: Human
• Form: The protein solution (0.7 mg/ml)contains 20 mM HEPES pH8, 100 mM NaCl, 20% Glycerol.
• Purity: Greater than 95.0% asdetermined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Endotoxin Level: Less than 0.1 ng/ug(IEU/ug) of recombinant Human GIF.
• Biological Activity: Standard ELISA test(checker-board analysis of positive/negative sera panels), immunodot withanti-IF autoantibody positive patient sera. Can be used for solid ELISA andfluid phase diagnostic assays, 0.25-0.65µg/ml. Suitable for biotinylation andiodination.
• Storage: GIF althoughstable at 10°C for 1 week, should be storeddesiccated below -18°C. For long termstorage it is recommended to add a carrier protein (0.1% HSA or BSA). Pleaseprevent freeze-thaw cycles.
• OfficialSymbol: GIF

Gene Information

• Gene Name: GIF gastric intrinsic factor(vitamin B synthesis) [ Homo sapiens ]
• Synonyms: GIF; gastric intrinsic factor(vitamin B synthesis); IF; INF; IFMH; TCN3; Gastric intrinsic factor;Intrinsic factor
• Gene ID: 2694
• mRNA Refseq: NM_005142
• Protein Refseq: NP_005133
• MIM: 609342
• UniProt ID: P27352
• Chromosome Location: 11
• Pathway: Vitamin digestionand absorption
• Function: cobalamin binding