Recombinant Human AKT1 Substrate 1 (proline-rich), His-tagged And Myc-tagged

• Cat.No.: AKT1S1-1854H
• Product Overview: Recombinant humanAKT1S1 containing amino acids 2-256 is produced as a maltose binding protein(MBP) fusion protein with an N-terminal polyhistidine tag and a C-terminalmyc Tag.

Specification

• Cat. No.: AKT1S1-1854H
• Description: AKT1S1 is a protein that in humans is encoded bythe AKT1S1 gene.
• Source: E. coli
• Species: Human
• Form: Lyophilized from a0.2 sterile filtered solution, carrier-free.
• Purity: > 90% asdetermined by SDS-PAGE analysis
• Molecular Weight: 75 kDa (migrates at85 kDa on SDS-PAGE gel)
• Biological Activity: Recombinant humanAKT1S1 is phosphorylatable in vitro, using either recombinant active Akt1, orwith immunoprecipitated Akt from stimulated cells. This phosphorylation canbe monitored by Western blot analysis using a phosphorylation site specificantibody directed to AKT1S1, in conjunction with chemiluminescence detectionmethods. Optimization of the cell stimulation protocol, cell lysis procedure,and reaction conditions may be required for each specific application.
• Reconstitution: Centrifuge vialprior to opening. Add PBS to the vial to fully solubilize the protein to aconcentration of 0.2-1.0 mg/ml.
• Storage: Store lyophilizedprotein at 2-4°C and reconstituted protein in working aliquots at -20°C to-80°C. Avoid repeated freeze-thaw cycles.
• OfficialSymbol: AKT1S1

Gene Information

• Gene Name: AKT1S1AKT1 substrate 1 (proline-rich) [ Homo sapiens ]
• Synonyms: AKT1S1; AKT1 substrate 1 (proline-rich); Lobe; PRAS40; MGC2865;AKT1S1; proline-rich AKT1 substrate 1; 40 kDa proline-rich AKT substrate;proline-rich Akt substrate, 40 kDa; OTTHUMP00000196759; OTTHUMP00000196760; OTTHUMP00000196756;OTTHUMP00000196757; OTTHUMP00000196758
• Gene ID: 84335
• mRNA Refseq: NM_001098632
• Protein Refseq: NP_001092102
• MIM: 610221
• UniProt ID: Q96B36
• Chromosome Location: 19q13.33
• Pathway: AKT phosphorylatestargets in the cytosol; Downstream signaling of activated FGFR; LKB1signaling events; NGF signalling via TRKA from the plasma membrane; PI-3Kcascade; PI3K/AKT activation; PIP3 activates AKT signaling; Signaling by FGFR;Signaling events mediated by Hepatocyte Growth Factor Receptor (c-Met); Signalling by NGF; mTOR signaling pathway
• Function: protein binding