Recombinant Human AKT1 Substrate 1 (proline-rich), His-tagged And Myc-tagged
Cat.No. : | AKT1S1-1854H |
Product Overview : | Recombinant humanAKT1S1 containing amino acids 2-256 is produced as a maltose binding protein(MBP) fusion protein with an N-terminal polyhistidine tag and a C-terminalmyc Tag. |
- Specification
- Gene Information
- Related Products
Cat. No. : | AKT1S1-1854H |
Description : | AKT1S1 is a protein that in humans is encoded bythe AKT1S1 gene. |
Source : | E. coli |
Species : | Human |
Form : | Lyophilized from a0.2 sterile filtered solution, carrier-free. |
Purity : | > 90% asdetermined by SDS-PAGE analysis |
Molecular Weight : | 75 kDa (migrates at85 kDa on SDS-PAGE gel) |
Biological Activity : | Recombinant humanAKT1S1 is phosphorylatable in vitro, using either recombinant active Akt1, orwith immunoprecipitated Akt from stimulated cells. This phosphorylation canbe monitored by Western blot analysis using a phosphorylation site specificantibody directed to AKT1S1, in conjunction with chemiluminescence detectionmethods. Optimization of the cell stimulation protocol, cell lysis procedure,and reaction conditions may be required for each specific application. |
Reconstitution : | Centrifuge vialprior to opening. Add PBS to the vial to fully solubilize the protein to aconcentration of 0.2-1.0 mg/ml. |
Storage : | Store lyophilizedprotein at 2-4°C and reconstituted protein in working aliquots at -20°C to-80°C. Avoid repeated freeze-thaw cycles. |
OfficialSymbol : | AKT1S1 |
Gene Name : | AKT1S1AKT1 substrate 1 (proline-rich) [ Homo sapiens ] |
Synonyms : | AKT1S1; AKT1 substrate 1 (proline-rich); Lobe; PRAS40; MGC2865;AKT1S1; proline-rich AKT1 substrate 1; 40 kDa proline-rich AKT substrate;proline-rich Akt substrate, 40 kDa; OTTHUMP00000196759; OTTHUMP00000196760; OTTHUMP00000196756;OTTHUMP00000196757; OTTHUMP00000196758 |
Gene ID : | 84335 |
mRNA Refseq : | NM_001098632 |
Protein Refseq : | NP_001092102 |
MIM : | 610221 |
UniProt ID : | Q96B36 |
Chromosome Location : | 19q13.33 |
Pathway : | AKT phosphorylatestargets in the cytosol; Downstream signaling of activated FGFR; LKB1signaling events; NGF signalling via TRKA from the plasma membrane; PI-3Kcascade; PI3K/AKT activation; PIP3 activates AKT signaling; Signaling by FGFR;Signaling events mediated by Hepatocyte Growth Factor Receptor (c-Met); Signalling by NGF; mTOR signaling pathway |
Function : | protein binding |
Products Types
◆ Recombinant Protein | ||
AKT1S1-444M | Recombinant Mouse AKT1S1 Protein, His (Fc)-Avi-tagged | +Inquiry |
Akt1s1-1585M | Recombinant Mouse Akt1s1 Protein, Myc/DDK-tagged | +Inquiry |
AKT1S1-27H | Recombinant Human AKT1S1 protein, MYC/DDK-tagged | +Inquiry |
AKT1S1-1934H | Recombinant Human AKT1S1, GST-tagged | +Inquiry |
AKT1S1-1508M | Recombinant Mouse AKT1S1 Protein | +Inquiry |
◆ Lysates | ||
AKT1S1-15HCL | Recombinant Human AKT1S1 lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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