Recombinant Mouse Fgf7 protein Fgf7-649M

Recombinant Mouse Fgf7 protein

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Recombinant Mouse Fgf7 protein

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Cat.No. : Fgf7-649M
Product Overview : Recombinant Mouse Fgf7 protein was expressed in Escherichia coli.
Description : Murine KGF-1 also known as Fibroblast growth factor 7 (FGF-7), is encoded by the FGF7 gene. KGF-1 only binds to the b splice form of the tyrosine kinase receptor, FGFR2b/KGFR. Affinity between KGF-1 and its receptor can be increased by heparin or heparan sulfate proteoglycan. FGF-10, also called keratinocyte growth factor 2 (KGF-2), shares 51 % amino acid sequence identity and similar function to KGF-1, but uses an additional receptor, FGFR2c. KGF-1 plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. KGF-1 actives on keratinocytes, and exhibits mitogenic activity for epidermal cells, but essentially no activity for fibroblasts. KGF-1 has species crossactive, murine KGF-1 shares 96 % amino acid sequence identity with human and rat.
Source : E.coli
Species : Mouse
Form : Lyophilized from a 0.2μm filtered solution in 20 mM PB, pH 8.0, 1 M NaCl.
Bio-activity : Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 10 ng/ml, corresponding to a specific activity of > 1.0 × 10⁵ IU/mg.
Molecular Mass : Approximately 18.7 kDa, a single, non-glycosylated polypeptide chain containing 163 amino acids.
Protein length : 163
AA Sequence : CNDMSPEQTA TSVNCSSPER HTRSYDYMEG GDIRVRRLFC RTQWYLRIDK RGKVKGTQEM KNSYNIMEIR TVAVGIVAIK GVESEYYLAM NKEGKLYAKK ECNEDCNFKE LILENHYNTY ASAKWTHSGG EMFVALNQKG IPVKGKKTKK EQKTAHFLPM AIT
Endotoxin : Less than 1 EU/µg of rMuKGF-1/FGF-7 as determined by LAL method.
Purity : >96% by SDS-PAGE and HPLC analysis.
Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution : We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions.
Gene Name : Fgf7
Official Symbol : Fgf7
Synonyms : FGF7; fibroblast growth factor 7; FGF-7; HBGF-7; Keratinocyte growth factor; heparin-binding growth factor 7; Kgf;
Gene ID : 14178
mRNA Refseq : NM_008008
Protein Refseq : NP_032034
UniProt ID : P36363
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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