| Cat. No. : |
Ngf-662M |
| Description : |
NGF is mainlyresponsible for the survival and the differentiation and the functionalactivities of sensory and sympathetic neurons in the peripheral nervous system.It also plays an important role in the development and functional activitiesof cholinergic neurons in the central nervous system. Since NGF issynthesized also in non-neuronal tissues it may have a much wider spectrum ofbiological activities than thought previously. NGF stimulates chemotacticmigration of human polymorphonuclear leukocytes in vitro. |
| Source : |
Mouse |
| Host : |
CHO |
| Purity : |
>95%, asdetermined by SDS-PAGE and HPLC. |
| Sequence : |
MSMLFYTLITAFLIGVQAEP YTDSNVPEGD SVPEAHWTKL QHSLDTALRR ARSAPTAPIA ARVTGQTRNI TVDPRLFKKRRLHSPRVLFS TQPPPTSSDT LDLDFQAHGT IPFNRTHRSK RSSTHPVFHM GEFSVCDSVS VWVGDKTTATDIKGKEVTVL AEVNINNSVF RQYFFETKCR ASNPVESGCR GIDSKHWNSY CTTTHTFVKA LTTDEKQAAWRFIRIDTACV CVLSRKATRR G |
| Molecular Weight : |
Native Mouse Ngf isgenerated by the proteolytic removal of the signal peptide and propeptide themolecule has a calculated molecular mass of approximately 27 kDa. |
| Biological Activity : |
TheED (50) was determined by the determined by its ability to stimulate chick E9DRG neurite outgrowth was found to be in the range of 1.0 ng/ml. |
| Endotoxin : |
Endotoxin contentwas assayed using a LAL gel clot method. Endotoxin level was found to be lessthan 0.1 ng/µg(1EU/µg). |
| Reconstitution : |
A quick spin of thevial followed by reconstitution in distilled water to a concentration notless than 0.1 mg/mL. This solution can then be diluted into other buffers. |
| Storage : |
The lyophilized protein is stable forat least 2 years from date of receipt at -20°C. Upon reconstitution, thiscytokine can be stored in working aliquots at 2°C - 8°C for one month, or at-20°C for six months, with a carrier protein without detectable loss ofactivity. Avoid repeated freeze/thaw cycles. |
| OfficialSymbol : |
Ngf |
| Pathways : |
ARMS-mediatedactivation; Activation of TRKA receptors; Apoptosis; Axonal growth stimulation;Ceramide signaling; Id Signaling Pathway; MAPK signaling pathway; NGFprocessing; Neurotrophin signaling pathway; PI3K/AKT activation; PLC-gamma1signalling; Signalling by NGF; Signalling to ERKs; Signalling to RAS; Signallingto STAT3 |
