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Recombinant Mouse Kit Ligand

Cat. No.: Kitlg-5252M
Product Overview: Recombinant Kitlg is a disulfide-linkedmonomer protein consisting of 175 amino acid residue subunits, and due toglycosylation migrates as an approximately 23 kDa protein under non-reducing andreducing conditions in SDS-PAGE, and was lyophilized from a 0.2 μm filteredsolution in 20mM Tris, 5% trehalose, pH 7.5.
Description: Kitlg is astromal cell-derived cytokine synthesized by fibroblasts and other celltypes. Kitlg promotes proliferation and early differentiation of cells at thelevel of multipotential stem cells. It has been suggested that Kitlg isessential for optimal production of various hematopoietic lineages, mainlybecause of its ability to prevent apoptosis when it co-stimulates with othercytokines. The receptor for Kitlg, designated SCFR(CD117), is the oncogenedesignated as KIT.
Source: Mouse
Host: Insectcells
Purity: >95%,as determined by SDS-PAGE and HPLC.
Sequence: MKKTQTWIIT CIYLQLLLFN PLVKTKEICG NPVTDNVKDI TKLVANLPND YMITLNYVAG MDVLPSHCWL RDMVIQLSLS LTTLLDKFSN ISEGLSNYSI IDKLGKIVDD LVLCMEENAP KNIKESPKRP ETRSFTPEEF FSIFNRSIDA FKDFMVASDT SDCVLSSTLG PEKDSRVSVT KPFMLPPVAA SSLRNDSSSS NRKAAKAPED SGLQWTAMAL PALISLVIGF AFGALYWKKK QSSLTRAVEN IQINEEDNEI SMLQQKEREF QEV
Molecular Weight: Native Mouse Stem Cell Factor is generatedby the proteolytic removal of the signal peptide and propeptide, the moleculehas a calculated molecular mass of approximately 18 kDa.
Biological Activity: TheED(50) was determined by the dose-dependent stimulation of the proliferationof human TF-1 cells is ≤ 2.0 ng/ml, corresponding to a specific activity of ≥5 x 105 units/mg.
Endotoxin: Endotoxin content was assayed using a LAL gel clotmethod. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Reconstitution: A quick spin of the vial followed by reconstitutionin distilled water to a concentration not less than 0.1 mg/mL. Thissolution can then be diluted into other buffers.
Storage: Thelyophilized protein is stable for at least 2 years from date of receipt at-20°C. Upon reconstitution, this cytokine can be stored in working aliquots at2°C - 8°C for one month, or at -20°C for six months, with a carrier proteinwithout detectable loss of activity. Avoid repeated freeze/thaw cycles.
OfficialSymbol: Kitlg
Pathways: Cytokine-cytokinereceptor interaction; Hematopoietic cell lineage; Kit Receptor SignalingPathway; Melanogenesis; Pathways in cancer
Gene Name: Kitlg KIT ligand [ Rattus norvegicus ]
Synonyms: Kitlg; kitligand; Mgf;SCF; Kitl; kit ligand; C-kit ligand; stem cell factor KL-1; mast cell growthfactor; steel factor/kit ligand; hematopoietic growth factor KL
Gene ID: 60427
mRNA Refseq: NM_021843
Protein Refseq: NP_068615
UniProt ID: P21581
Function: cytokine activity; growthfactor activity; identical protein binding; stem cell factor receptor binding

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