||Recombinanthuman RDH12protein, fused to His-tag at N-terminus, was expressed in E.coli andpurified by using conventional chromatography techniques.
||RDH12 belongs to theshort-chain dehydrogenases /reductases (SDR) family. RDH12 is expressed,mostly in eye, kidney, brain, skeletal muscle and stomach. It is anNADPH-dependent retinal reductase whose highest activity is toward 9-cis andall-trans-retinol. RDH12 also plays a role in the metabolism of short-chainaldehydes but does not exhibit steroid dehydrogenase activity.
||Liquid. In 20 mMTris-HCl buffer (pH 8.0) containing 2 mM DTT, 40% glycerol, 0.2M NaCl.
||33.5 kDa(303 aa), confirmed by MALDI-TOF.
||> 90%by SDS-PAGE
||0.25mg/ml(determined by Bradford assay)
|Sequences of aminoacids:
||MGSSHHHHHHSSGLVPRGSH MGSHMGKVVV ITGANTGIGK ETARELASRG ARVYIACRDVLKGESAASEI RVDTKNSQVL VRKLDLSDTK SIRAFAEGFLAEEKQLHILI NNAGVMMCPY SKTADGFETH LGVNHLGHFL LTYLLLERLK VSAPARVVNV SSVAHHIGKIPFHDLQSEKR YSRGFAYCHS KLANVLFTRE LAKRLQGTGV TTYAVHPGVV RSELVRHSSL LCLLWRLFSPFVKTAREGAQ TSLHCALAEG LEPLSGKYFS DCKRTWVSPRARNNKTAERL WNVSCELLGI RWE
||Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
||Metabolicpathways; Retinol metabolism; Visual signal transduction; Vitamin A andcarotenoid metabolism