||Recombinant humanLASP1 protein, fused to His-tag at N-terminus, was expressed in E. coli andpurified by using conventional chromatography.
||LASP1, also known asLIM and SH3 domain protein 1, plays an important role in the regulation ofdynamic actin-based, cytoskeletal activities. Agonist-dependent changes inLASP1 phosphorylation may also serve to regulate actin-associated ion transportactivities, not only in the parietal cell but also in certain otherF-actin-rich secretory epithelial cell types.
||Liquid. 20mMTris-HCl buffer (pH8.0) containing 20% glycerol, 1mM DTT.
||32.3 kDa(285aa), confirmed by MALDI-TOF.
||> 80%by SDS - PAGE
||0.25mg/ml(determined by Bradford assay)
|Sequences of aminoacids:
||MGSSHHHHHHSSGLVPRGSH MGSHMNPNCA RCGKIVYPTE KVNCLDKFWH KACFHCETCK MTLNMKNYKGYEKKPYCNAH YPKQSFTMVA DTPENLRLKQ QSELQSQVRY KEEFEKNKGK GFSVVADTPE LQRIKKTQDQISNIKYHEEF EKSRMGPSGG EGMEPERRDS QDGSSYRRPL EQQQPHHIPTSAPVYQQPQQ QPVAQSYGGY KEPAAPVSIQ RSAPGGGGKR YRAVYDYSAA DEDEVSFQDG DTIVNVQQIDDGWMYGTVERTGDTGMLPANYVEAI
||Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.