Recombinant Human Carbonic Anhydrase VIII, His-tagged

• Cat.No.: CA8-1388H
• Product Overview: Recombinant humanCA8 protein fused to His-tag at N-terminus, was expressed in E. coli andpurified by using conventional chromatography.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: CA8 wasinitially named CA-related protein because of sequence similarity to otherknown carbonic anhydrase genes. However, this protein lacks carbonicanhydrase activity (i.e., the reversible hydration of carbon dioxide). Itcontinues to carry a carbonic anhydrase designation based on clear sequenceidentity to other members of the carbonic anhydrase gene family. Defects inCA8 are the cause of cerebellar ataxia mental retardation and dysequilibriumsyndrome type 3 (CMARQ3).
• Form: Liquid. 20mM Tris-HClbuffer (pH8.0) containing 20% glycerol, 1mM DTT.
• Molecular Weight: 35.5 kDa(314aa), confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appearhigher).
• Purity: > 90%by SDS - PAGE
• Concentration: 1 mg/ml(determined by Bradford assay)
• Sequences of aminoacids: MGSSHHHHHHSSGLVPRGSH MGSHMADLSF IEDTVAFPEK EEDEEEEEEG VEWGYEEGVE WGLVFPDANGEYQSPINLNS REARYDPSLL DVRLSPNYVV CRDCEVTNDG HTIQVILKSK SVLSGGPLPQ GHEFELYEVRFHWGRENQRG SEHTVNFKAF PMELHLIHWN STLFGSIDEA VGKPHGIAII ALFVQIGKEH VGLKAVTEILQDIQYKGKSK TIPCFNPNTL LPDPLLRDYW VYEGSLTIPP CSEGVTWILF RYPLTISQLQ IEEFRRLRTHVKGAELVEGC DGILGDNFRP TQPLSDRVIR AAFQ
• Storage: Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: CA8 carbonic anhydrase VIII [ Homosapiens ]
• Official Symbol: CA8
• Synonyms: CA8; carbonic anhydrase VIII; CALS; CARP;CAMRQ3; CA-VIII; MGC99509; MGC120502; carbonic anhydrase-related protein;CA-related protein; OTTHUMP00000229370; carbonate dehydratase; carbonicanhydrase-like sequence; EC 4.2.1.1
• Gene ID: 767
• mRNA Refseq: NM_004056
• Protein Refseq: NP_004047
• MIM: 114815
• UniProt ID: P35219
• Chromosome Location: 8q11-q12
• Pathway: Nitrogen metabolism
• Function: carbonate dehydratase activity; metal ionbinding; metal ion binding