Recombinant Human Carbonic Anhydrase VIII, His-tagged

• Cat.No.: CA8-1388H
• Product Overview: Recombinant humanCA8 protein fused to His-tag at N-terminus, was expressed in E. coli andpurified by using conventional chromatography.

Specification

• Cat. No.: CA8-1388H
• Description: CA8 wasinitially named CA-related protein because of sequence similarity to otherknown carbonic anhydrase genes. However, this protein lacks carbonicanhydrase activity (i.e., the reversible hydration of carbon dioxide). Itcontinues to carry a carbonic anhydrase designation based on clear sequenceidentity to other members of the carbonic anhydrase gene family. Defects inCA8 are the cause of cerebellar ataxia mental retardation and dysequilibriumsyndrome type 3 (CMARQ3).
• Form: Liquid. 20mM Tris-HClbuffer (pH8.0) containing 20% glycerol, 1mM DTT.
• Molecular Weight: 35.5 kDa(314aa), confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appearhigher).
• Purity: > 90%by SDS - PAGE
• Concentration: 1 mg/ml(determined by Bradford assay)
• Sequences of aminoacids: MGSSHHHHHHSSGLVPRGSH MGSHMADLSF IEDTVAFPEK EEDEEEEEEG VEWGYEEGVE WGLVFPDANGEYQSPINLNS REARYDPSLL DVRLSPNYVV CRDCEVTNDG HTIQVILKSK SVLSGGPLPQ GHEFELYEVRFHWGRENQRG SEHTVNFKAF PMELHLIHWN STLFGSIDEA VGKPHGIAII ALFVQIGKEH VGLKAVTEILQDIQYKGKSK TIPCFNPNTL LPDPLLRDYW VYEGSLTIPP CSEGVTWILF RYPLTISQLQ IEEFRRLRTHVKGAELVEGC DGILGDNFRP TQPLSDRVIR AAFQ
• Storage: Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: CA8 carbonic anhydrase VIII [ Homosapiens ]
• Official Symbol: CA8
• Synonyms: CA8; carbonic anhydrase VIII; CALS; CARP;CAMRQ3; CA-VIII; MGC99509; MGC120502; carbonic anhydrase-related protein;CA-related protein; OTTHUMP00000229370; carbonate dehydratase; carbonicanhydrase-like sequence; EC 4.2.1.1
• Gene ID: 767
• mRNA Refseq: NM_004056
• Protein Refseq: NP_004047
• MIM: 114815
• UniProt ID: P35219
• Chromosome Location: 8q11-q12
• Pathway: Nitrogen metabolism
• Function: carbonate dehydratase activity; metal ionbinding; metal ion binding

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Q&As

Are there specific proteins or molecules known to interact with CA8?

CA8 interacts with IP3Rs, affecting calcium signaling in neurons.

How does CA8's role differ from the enzymatically active carbonic anhydrase isoforms?

Unlike enzymatically active isoforms, CA8 doesn't catalyze the hydration of CO2 but instead plays roles in neuronal signaling and function.

Are there genetic mutations in the CA8 gene that are linked to specific diseases?

Mutations in CA8 have been linked to cerebellar ataxia, a movement disorder.

How does CA8 contribute to cellular processes, given its non-enzymatic nature?

CA8 impacts calcium signaling in neurons, affecting neural communication and potentially influencing motor control.

What post-translational modifications are typical for CA8?

Specific modifications, like phosphorylation, can be explored using mass spectrometry or targeted biochemical assays.

What is the primary function of CA8, considering it's a non-enzymatic member of the carbonic anhydrase family?

CA8 acts as a stabilizer for inositol 1,4,5-trisphosphate receptors (IP3Rs) in neurons.

In which human tissues or cell types is CA8 predominantly expressed?

CA8 is mainly expressed in the cerebellum, particularly in Purkinje cells.