||Recombinant human ABHD14B protein, fused to His-tag atN-terminus, was expressed in E. coli andpurified by using conventional chromatography techniques.
||ABHD14B(abhydrolase domain containing 14B) belongs to the AB hydrolase superfamily.This protein contains an alpha/beta hydrolase fold, which is acatalytic domain found in a very wide range of enzymes. In molecular biology,the alpha/beta hydrolase fold is common to a number of hydrolytic enzymes ofwidely differing phylogenetic origin and catalytic function. The Ab hydrolase domain containing (ABHD) gene subfamily iscomprised of 15 mostly uncharacterized members. ABHD14B has hydrolaseactivity towards p-nitrophenyl butyrate (in vitro). It may activatetranscription.
||Liquid. In 20mMTris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 100mM NaCl.
||25 kDa(234aa), confirmed by MALDI-TOF.
||> 95%by SDS-PAGE
||0.5mg/ml(determined by Bradford assay)
|Sequences of aminoacids:
||MGSSHHHHHHSSGLVPRGSH MGSHMAASVE QREGTIQVQG QALFFREALP GSGQARFSVL LLHGIRFSSETWQNLGTLHR LAQAGYRAVA IDLPGLGHSK EAAAPAPIGE LAPGSFLAAV VDALELGPPV VISPSLSGMYSLPFLTAPGS QLPGFVPVAP ICTDKINAAN YASVKTPALI VYGDQDPMGQ TSFEHLKQLP NHRVLIMKGAGHPCYLDKPE EWHTGLLDFL QGLQ
||Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.