Recombinant Human PTK-2 Protein Tyrosine Kinase 2, His-tagged


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Recombinant Human PTK-2 Protein Tyrosine Kinase 2, His-tagged

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Cat. No. : PTK2-1901H
Product Overview : Recombinant Human PTK2 (a.a. 411-686) with N-terminal His tag, MW 36kDa, expressed in an Baculovirus infected Sf9 cell expression system.
Description : PTK2 is a cytoplasmic proteintyrosine kinase which is found concentrated in the focal adhesions.Activation of this gene may be an important early step in cell growth andintracellular signal transduction pathways triggered in response to certainneural peptides or to cell interactions with the extracellular matrix.
Source : Baculovirus infectedSf9 cells
Species : Human
Form : 25 mM Tris-HCl, pH8.0 + 100 mM NaCl + 0.05% Tween-20 + 50% glycerol + 3 mM DTT.
Purity : > 95% by SDS-PAGE
Specific Activity : 100 U/mg. One unitis defined as the amount of enzyme that will phosphorylate 1 nmol of tyrosinesubstrate per minute at pH 7.4 and 30°C. Assay buffer: 50 mM HEPES, pH 7.4 +3 mM MgCl2 + 3 mM MnCl2 + 1 mM DTT + 3 ugNa-orthovanadate + 0.1 mM ATP + 30 ug/ml Poly (Glu:Tyr)4:1 substrate + 1 ug/ml recombinant PTK2.
Storage : > 6 months at -80°C.
OfficialSymbol : PTK2
Gene Name : PTK2 PTK2 protein tyrosinekinase 2 [ Homo sapiens ]
Synonyms : PTK2; PTK-2 protein tyrosinekinase 2; FAK; FADK; FAK1; pp125FAK; Focal adhesion kinase 1; FADK 1;Protein-tyrosine kinase 2; Protein-tyrosine kinase 2; EC
Gene ID : 5747
mRNA Refseq : NM_005607
Protein Refseq : NP_005598
MIM : 600758
UniProt ID : Q05397
Chromosome Location : 8q24
Pathway : Axon guidance; ErbB signalingpathway; Chemokine signaling pathway; Focal adhesion; Leukocytetransendothelial migration; Pathways in cancer; Regulation of actincytoskeleton; Small cell lung cancer; VEGF signaling pathway; Apoptosis; Axonguidance; Hemostasis; Integrin cell surface interactions
Function : ATP binding; SH2 domainbinding; non-membrane spanning protein tyrosine kinase activity; nucleotidebinding; protein binding; signal transducer activity; transferase activity

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