Recombinant Human PTK2B Protein Tyrosine Kinase 2 Beta, His-tagged

• Cat.No.: PTK2B-1904H
• Product Overview: Recombinant humanfull length PTK2B with N-terminal His tag.

Specification

• Cat. No.: PTK2B-1904H
• Description: PTK2B is a cytoplasmic tyrosine kinaseinvolved in calcium-induced regulation of ion channels and activation of themap kinase signaling pathway. No special measures were taken to activate thiskinase.
• Source: Sf9 insect cells
• Species: Human
• Form: Liquid in 25 mMTris-HCl, pH 8.0 + 100 mM NaCl + 0.05% Tween-20 + 50% glycerol + 3 mM DTT.
• Molecular Weight: 120.0 kDa
• Purity: > 90% asdetermined by SDS-PAGE analysis
• Biological Activity: 20 U/mg. One unit isdefined as the amount of enzyme that will phosphorylate 1 nmol of tyrosinesubstrate per minute at pH 7.4 and at 30°C. Assay buffer: 50 mM HEPES, pH 7.4+ 3 mM MgCl2 + 3 mM MnCl2 + 1 mM DTT + 3 mMNa-orthovanadate + 0.5 mM ATP + 30 ug/ml Poly (Glu:Tyr) 4:1 substrate + 4 ug/mlrecombinant PTK2B.
• Storage: Stablefor 6 months in working aliquots at -80°C. Avoid repeated freeze-thaw cycles.
• OfficialSymbol: PTK2B

Gene Information

• Gene Name: PTK2B PTK2B protein tyrosinekinase 2 beta [ Homo sapiens ]
• Synonyms: PTK2B; PTK2B protein tyrosinekinase 2 beta; PTK2B protein tyrosine kinase 2 beta; CAK beta;calcium-dependent tyrosine kinase; cell adhesion kinase beta; focal adhesionkinase 2; proline-rich tyrosine kinase 2; protein kinase B; protein tyrosinekinase 2 beta; related adhesion focal tyrosine kinase; EC 2.7.10.2
• Gene ID: 2185
• mRNA Refseq: NM_004103
• Protein Refseq: NP_004094
• MIM: 601212
• UniProt ID: Q14289
• Chromosome Location: 8p21.1
• Pathway: Calcium signaling pathway;Chemokine signaling pathway; GnRH signaling pathway; Leukocytetransendothelial migration; Natural killer cell mediated cytotoxicity
• Function: 3-phosphoinositide-dependentprotein kinase binding; ATP binding; non-membrane spanning protein tyrosinekinase activity; nucleotide binding; protein complex binding; signaltransducer activity; transferase activity