Recombinant Human Protein Tyrosine Phosphatase, Non-Receptor Type 12

• Cat.No.: PTPN12-1909H
• Product Overview: Recombinanthuman PTPN12 is expressed in E. coli; it contains the catalytic domain ofPTPN12 (residues 2-300) and has a molecular weight of 35.4 kDa.

Specification

• Cat. No.: PTPN12-1909H
• Description: PTPN12 (protein tyrosine phosphatase-PEST)is a cytosolic protein tyrosine phosphatase belonging to the PEP family. PTPN12is expressed ubiquitously, but in higher levels in hemopoietic tissues.Full-length PTPN12 has been shown to associate with proteins such as Shc,paxillin, Hic-5, Cas, CasL, Sin, and Csk, which all have roles in signaltransduction. PTPN12 has also been implicated in the regulation of cellmotility through it"s interaction with Rac1. Human PTPN12 is a proteintyrosine phosphatase containing 780 amino acids.
• Source: E. coli
• Species: Human
• Form: 25 mM Tris-HCl (pH 8.0), 75 mM NaCl,0.05% Tween-20, 2 mM EDTA, 1 mM DTT and 50% glycerol. Liquid at -20°C.
• Purity: Greaterthan 95% as determined by Coomassie-stained SDS-PAGE gel.
• Specific Activity: 1.7 U/μg. One unitwill hydrolyze 1 nmol of p-nitrophenyl phosphate per minute at pH 7.4 and30°C. Assay buffer: 50 mM HEPES (pH 7.4), 2 mM EDTA, 3 mM DTT, 100 mM NaCland 50 mM pNPP.
• Applications: Removal ofphosphates from target substrates. Useful for the study of enzyme kinetics,regulation and for inhibitors screening.
• Storage: 6 months at -20°C.
• Pathways: EGFR1 SignalingPathway; T Cell Receptor Signaling Pathway

Gene Information

• Gene Name: PTPN12 protein tyrosinephosphatase, non-receptor type 12 [ Homo sapiens ]
• Official Symbol: PTPN12
• Synonyms: PTPN12; proteintyrosine phosphatase, non-receptor type 12; PTPG1; PTP-PEST;tyrosine-protein phosphatase non-receptor type 12; OTTHUMP00000161275; OTTHUMP00000208615;OTTHUMP00000208616; protein-tyrosine phosphatase G1
• Gene ID: 5782
• mRNA Refseq: NM_001131008
• Protein Refseq: NP_001124480
• MIM: 600079
• UniProt ID: Q05209
• Chromosome Location: 7q11.23
• Function: SH3 domain binding;hydrolase activity; non-membrane spanning protein tyrosine phosphataseactivity; protein binding