Recombinant Mouse Vascular Endothelial Growth Factor A
Cat.No. : | Vegfa-5533M |
Product Overview : | Recombinant mouse Vegfais produced in serum-free adapted Sf9 cells and purified via sequentialchromatography. |
- Specification
- Gene Information
- Related Products
Cat. No. : | Vegfa-5533M |
Description : | This gene is amember of the platelet-derived growth factor (PDGF)/vascular endothelialgrowth factor (VEGF) family and encodes a protein that is often found as adisulfide linked homodimer. This protein is a glycosylated mitogen thatspecifically acts on endothelial cells and has various effects. |
Source : | Sf9 cells |
Species : | Mouse |
Form : | Lyophilized, carrierfree. |
Purity : | > 95% asdetermined by SDS-PAGE analysis. |
Molecular Weight : | 38.8 kDa(homodimer), 164 amino acid residues/subunit. |
Endotoxin Level : | < 0.1 ng/μg |
Biological Activity : | ED50range 1-6 ng/ml, determined by the dose dependent proliferation of humanumbilical vein cells (HUVEC). Optimal concentration for individualapplication should be determined by a dose response assay. |
Sterility : | Filtered prior tolyophilization through a 0.22 micron sterile filter. |
Reconstitution : | We recommend thatthis vial be briefly centrifuged prior to opening to bring the contents tothe bottom. Lyophilized mVEGF should be reconstituted to 0.1-1.0 mg/ml insterile deionized water or appropriate buffered solution containing 0.1% BSAto regain full activity. These stock solutions should be apportioned intoworking aliquots and stored at ≤-20oC. Further dilutions should be made inlow endotoxin medium or buffered solution with FBS or tissue culture gradeBSA. The optimal concentration should be determined for each specificapplication. |
Storage : | Lyophilized mVEGFshould be stored at 2-8°C, preferably desiccated. Store reconstituted mVEGFat ≤ -20°C (not in a frost-free freezer). Keep freeze-thaw cycles to aminimum. |
OfficialSymbol : | Vegfa |
Pathways : | Bladder cancer; Cytokine-cytokinereceptor interaction; Endochondral Ossification; Focal Adhesion; Focaladhesion; Hemostasis; Hypertrophy Model; Id Signaling Pathway; Neurophilininteractions with VEGF and VEGFR; PI3K/Akt/mTOR signaling pathway; Pancreaticcancer; Pathways in cancer; Platelet activation; Platelet degranulation; Renalcell carcinoma |
Gene Name : | Vegfa vascular endothelialgrowth factor A [ Mus musculus ] |
Synonyms : | Vegfa;vascular endothelial growth factor A; Vpf; Vegf; Vegf120; Vegf164; Vegf188; vascularendothelial growth factor A; OTTMUSP00000017463; OTTMUSP00000017464;OTTMUSP00000022243; OTTMUSP00000022244; OTTMUSP00000022245; vascularpermeability factor; Vascular endothelial growth factor A; Vascular permeability factor |
Gene ID : | 22339 |
mRNA Refseq : | NM_001025250 |
Protein Refseq : | NP_001020421 |
UniProt ID : | Q00731 |
Chromosome Location : | 17 C; 17 24.2 cM |
Function : | cell surface binding;chemoattractant activity; cytokine activity; fibronectin binding; growthfactor activity; heparin binding; platelet-derived growth factor receptorbinding; protein heterodimerization activity; protein homodimerizationactivity; vascular endothelial growth factor receptor 1 bindingvascularendothelial growth factor receptor 2 binding; vascular endothelial growthfactor receptor binding |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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