"KRT14" Related Products


Recombinant Human Keratin 14

Cat. No.: KRT14-374H
Product Overview: Recombinant humanKRT14 is a single, non-glycosylated polypeptide chain.
Description: KRT14, type I cytoskeletal 14 also known ascytokeratin-14 (CK-14) or keratin-14 (K14) is a protein that in humans isencoded by the KRT14 gene. Keratin 14 is a type I keratin. It is usuallyfound as a heterotetramer with two keratin 5 molecules, a type II keratin.Together they form the cytoskeleton of epithelial cells.
Source: E. coli
Species: Human
Form: Lyophilized from asterile filtered solution containing 30 mM Tris-HCl, pH 8.0 + 9.5 M urea + 2mM EDTA + 10 mM methylammonium chloride.
Molecular Weight: 51.53 kDa
Purity: > 95% asdetermined by SDS-PAGE and RP-HPLC analyses.
Endotoxin Level: < 0.1 ng/µg ofKRT14.
Reconstitution: Centrifuge vialprior to opening. First add sterile distilled water to the vial to fully solubilizethe protein to a concentration not less than 100 µg/ml. After complete solubilizationof the protein, it can be further diluted to other aqueous solutions.
Storage: Store lyophilizedprotein at -20°C to -80°C. Reconstituted protein is stable for 1 week at 2-4°C.For long term storage, aliquot and store at -20°C to -80°C with a carrierprotein (0.1% HSA or BSA) as a stabilizer. Please note that the addition ofany carrier protein into this product may produce unwanted endotoxin. Thisdepends upon the particular application employed. Avoid repeated freeze-thawcycles.
OfficialSymbol: KRT14
Gene Name: KRT14 keratin 14 [ Homosapiens ]
Synonyms: KRT14; keratin 14;K14; NFJ; CK14; EBS3; EBS4; keratin, type I cytoskeletal 14; CK-14;keratin-14; cytokeratin 14; Cytokeratin-14; cytokeratin-14; Keratin-14;OTTHUMP00000164624; keratin 14 (epidermolysis bullosa simplex, Dowling-Meara,Koebner)
Gene ID: 3861
mRNA Refseq: NM_000526
Protein Refseq: NP_000517
MIM: 148066
UniProt ID: P02533
Chromosome Location: 17q12-q21
Pathway: Glucocorticoidreceptor regulatory network
Function: protein binding;structural constituent of cytoskeleton

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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