||Recombinant humanfull length HCK with C-terminal His tag. No special measures were taken toactivate this kinase.
||Theprotein encoded by this gene is a HCK that ispredominantly expressed in hemopoietic cell types, and belongs to the Srcfamily of tyrosine kinases. The encoded protein may help couple the Fcreceptor to the activation of the respiratory burst. In addition, it may playa role in neutrophil migration and in the degranulation of neutrophils.Alternate translation initiation site usage, including a non-AUG (CUG) codon,results in the production of two different isoforms, that have differentsubcellular localization.
||Liquid in 50 mMTris, pH 7.5 + 0.05 mM EDTA + 1 mM DTT + 100 mM NaCl + 0.05% NP-40 + 50%glycerol.
||1450 nmole ofphosphate transferred to poly [Glu,Tyr] 4:1 substrate per minute per mg oftotal protein at 30°C. Activity determined at a final protein concentrationof 2 µg/ml.
||Stable for 6 monthsin working aliquots at -80°C. Avoid repeated freeze-thaw cycles.