||Recombinanthuman full length MAP3K11, GST-tagged, is expressed in Insect cells.
||MAP3K11is a serine/threonine kinase that activates both the p38 MAP kinase and c-Junkinase pathways. The protein encoded by this gene is a member of theserine/threonine kinase family. This kinase contains a SH3 domain and aleucine zipper-basic motif. This kinase preferentially activates MAPK8/JNKkinase, and functions as a positive regulator of JNK signaling pathway. Thiskinase can directly phosphorylate, and activates IkappaB kinase alpha andbeta, and is found to be involved in the transcription activity of NF-kappaBmediated by Rho family GTPases and CDC42.
||Liquid in50 mM Tris, pH 7.5 + 150 mM NaCl + 0.5 mM EDTA + 0.02% Triton X-100 + 2 mMDTT + 50% glycerol.
||43 nmoleof phosphate transferred to myelin basic protein (MBP) per minute per mg of totalprotein at 30°C. Activity determined at a final protein concentration of 8.33μg/ml.
||Stablefor 6 months in working aliquots at -80°C. Avoid repeated freeze-thaw cycles.
|Function||ATP binding; JUNkinase kinase kinase activity; Eac GTPase binding; identical protein binding;mitogen-activated protein kinase kinase kinase binding; mitogen-activatedprotein kinase kinase binding; protein kinase activity; nucleotide binding; proteinserine/tyreonine kinase activity; protein homodimerization activity; proteintyrosine kinase activity|