Recombinant Human Mitogen-Activated Protein Kinase Kinase Kinase 5, GST-tagged

• Cat.No.: MAP3K5-1260H
• Product Overview: RecombinantHuman MAP3K5 with N-terminal GST tag. MAP3K5 is a serine/threonine kinasethat is abundantly expressed in human heart and pancreas.

Specification

• Cat. No.: MAP3K5-1260H
• Description: The MAP3K5protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro,and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase(SAPK) during transient expression in COS and 293 cells; MAP3K5 does notactivate MAPK/ERK.
• Source: Insect cells
• Species: Human
• Form: Liquid in50 mM Tris, pH 7.5 + 150 mM NaCl + 0.5 mM EDTA + 0.02% Triton X-100 + 2 mMDTT + 50% glycerol.
• Molecular Weight: 182 kDa
• Specific Activity: 64 nmoleof phosphate transferred to myelin basic protein (MBP) per minute per mg of totalprotein at 30°C. Activity determined at a final protein concentration of 8.33μg/ml.
• Concentration: 0.25mg/ml
• Storage: Stablefor 6 months in working aliquots at -80°C. Avoid repeated freeze-thaw cycles.
• OfficialSymbol: MAP3K5
• Function: MAP kinase kinasekinase activity; metal ion binding; protein kinase activity; nucleotidebinding; protein serine/tyreonine kinase activity; protein phosphatase binding;protein homodimerization activity; protein binding; magnesium ion binding; cysteine-typeendopeptidase activator activity involved in apoptotic process

Gene Information

• Gene Name: MAP3K5 mitogen-activated proteinkinase kinase kinase 5 [ Homo sapiens ]
• Synonyms: MAP3K5; mitogen-activatedprotein kinase kinase kinase 5; MEKK5; MAPKKK5; ASK1; apoptosissignal regulating kinase 1; OTTHUMP00000017275; EC 2.7.11.25; ASK-1; MEKkinase 5; MAPK/ERK kinase kinase 5; MAP/ERK kinase kinase 5; apoptosis signal-regulatingkinase 1
• Gene ID: 4217
• mRNA Refseq: NM_005923
• Protein Refseq: NP_005914
• MIM: 602448
• UniProt ID: Q99683
• Chromosome Location: 6q22.33
• Pathway: p38 MAPK signalingpathway; Protein processing in endoplasmic reticulum; Class I PI3K signalingevents mediated by Akt; Amyotrophic lateral sclerosis (ALS); HTLV-Iinfection;IL1-mediatedsignaling events; Neurotrophin signaling pathway; InsulinSignaling; TNFreceptor signaling pathway; MAPK Cascade; MAPK signaling pathway; TNF-alpha/NF-kBSignaling Pathway; Trk receptor signaling mediated by the MAPK pathway