||RecombinantHuman MAP3K5 with N-terminal GST tag. MAP3K5 is a serine/threonine kinasethat is abundantly expressed in human heart and pancreas.
||The MAP3K5protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro,and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase(SAPK) during transient expression in COS and 293 cells; MAP3K5 does notactivate MAPK/ERK.
||Liquid in50 mM Tris, pH 7.5 + 150 mM NaCl + 0.5 mM EDTA + 0.02% Triton X-100 + 2 mMDTT + 50% glycerol.
||64 nmoleof phosphate transferred to myelin basic protein (MBP) per minute per mg of totalprotein at 30°C. Activity determined at a final protein concentration of 8.33μg/ml.
||Stablefor 6 months in working aliquots at -80°C. Avoid repeated freeze-thaw cycles.
|Function||MAP kinase kinasekinase activity; metal ion binding; protein kinase activity; nucleotidebinding; protein serine/tyreonine kinase activity; protein phosphatase binding;protein homodimerization activity; protein binding; magnesium ion binding; cysteine-typeendopeptidase activator activity involved in apoptotic process|