||Recombinant human full length MAP4K2,His-tagged is expressed in Insect cells.
||MAP4K2 is an enzymethat in humans is encoded by the MAP4K2 gene. The protein encoded by thisgene is a member of the serine/threonine protein kinase family. Although thiskinase is found in many tissues, its expression in lymphoid follicles isrestricted to the cells of germinal centre, where it may participate inB-cell differentiation. This kinase can be activated by TNF-alpha, and hasbeen shown to specifically activate MAP kinases. This kinase is also found tointeract with TNF receptor-associated factor 2 (TRAF2), which is involved inthe activation of MAP3K1/MEKK1.
||Liquid in50 mM Tris, pH 7.5 + 150 mM NaCl + 0.5 mM EDTA + 0.02% Triton X-100 + 2 mMDTT + 50% glycerol.
||728 nmole of phosphate transferred tomyelin basic protein (MBP) per minute per mg of total protein at 30°C.Activity determined at a final protein concentration of 3.33 μg/ml.
||Stablefor 6 months in working aliquots at -80°C. Avoid repeated freeze-thaw cycles.
|Function||ATP binding; mitogen-activatedprotein kinase kinase kinase binding; nucleotide binding; protein serine/tyreoninekinase activity; protein binding; small GTPase regulator activity|