||Human PRKCIexpressed in a Baculovirus infected Sf9 cell expression system.
||The PKCfamily of serine/threonine kinases, including PRKCI, is activatedintracellularly by signal transduction pathways. In humans, at least 12different PKC polypeptides have been identified. These isoforms differ inprimary structure, tissue distribution, subcellular localization, mode ofaction in vitro, response to extracellular signals, and substratespecificity. PKC alpha, beta I, beta II and gamma form the conventional family;their activities are Ca2+ and phospholipiddependent. No specialmeasures were taken to activate this kinase.
||25 mMTris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, 3 mM DTT.
||Greaterthan 95% by SDS-PAGE
||Usefulfor the study of enzyme kinetics, screening inhibitors and selectivity profiling.
||35 U/μg.One unit is defined as the amount of enzyme that will phosphorylate 1 pmol ofSer/Thr 1 peptide substrate per minute at pH 7.4 and 30°C. Assay buffer: 50mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3μM Na-orthovanadate, 0.5 mM ATP, 4 μM PKC substrate, lipid activator and0.2μg PKC.
||6 monthsat -80°C.
|Function||ATP binding; nucleotide binding;protein binding; protein kinase activity; protein serine/threonine kinaseactivity; zinc ion binding; metal ion binding; histone kinase activity (H3-T6specific); phospholopid binding|