Recombinant Human PRKCI Protein, GST-tagged

• Cat.No.: PRKCI-757H
• Product Overview: Recombinant Human PRKCI Protein(NP_002731.4) (Met 10-Val 596) was expressed in Insect Cells, fused with the GST tag at the C-terminus.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST
• Protein Length: 10-596 aa
• Form: Lyophilized from sterile 50mM Tris, 100mM NaCl, 0.5mM GSH, 0.5mM PMSF, 10% glycerol, pH 7.4.
• Molecular Mass: The recombinant human PKCλ/GST chimera consists of 812 amino acids and has a predicted a molecular mass of 93.5 kDa. It migrates as an approximately 100 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 88 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: PRKCI protein kinase C, iota [ Homo sapiens ]
• Official Symbol: PRKCI
• Synonyms: PRKCI; protein kinase C, iota; DXS1179E; protein kinase C iota type; PKCI; PRKC-lambda/iota; aPKC-lambda/iota; atypical protein kinase C-lambda/iota; nPKC-iota; MGC26534
• Gene ID: 5584
• mRNA Refseq: NM_002740
• Protein Refseq: NP_002731
• MIM: 600539
• UniProt ID: P41743